Superantigen-mediated differentiation of bovine monocytes into dendritic cells

Keun Seok Seo; Joo Youn Park; William C Davis; Lawrence K Fox; Mark A McGuire; Yong Ho Park; Gregory A Bohach

doi:10.1189/jlb.0608338

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Superantigen-mediated differentiation of bovine monocytes into dendritic cells

Journal article

Open access

Peer reviewed

Superantigen-mediated differentiation of bovine monocytes into dendritic cells

Keun Seok Seo, Joo Youn Park, William C Davis, Lawrence K Fox, Mark A McGuire, Yong Ho Park and Gregory A Bohach

Journal of leukocyte biology, Vol.85(4), pp.606-616

04/2009

DOI: https://doi.org/10.1189/jlb.0608338

Handle:

https://hdl.handle.net/2376/105601

PMCID: PMC2656427

PMID: 19129485

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Abstract

autologous MLR

staphylococcal enterotoxin C1

chemokines

Cell Development, Growth, Differentiation, and Trafficking

leukocyte trafficking

Although many effects of staphylococcal superantigens (SAg) on T cells are well established, less is known about their effects on APC. In this study, bovine PBMC were stimulated with a low dose of staphylococcal enterotoxin C1 (SEC1). The phenotype of adherent cells (Ac) derived from bovine PBMC cultured with SEC1 [SEC1-stimulated Ac (sAc)] for 192 h was CD14 − , CD68 − , CD163 − , dendritic cell (DC)-specific ICAM-3-grabbing nonintegrin + , MHC class II (MHC II) high , CD11a low , CD11b high , CD11c high , and CD1b high , suggesting these cells were dendritic cells (DC). SEC1 also induced transcription of the CXCL1, -2, and -3 family, CXCL6, CCL2, and CCL5 genes in sAc, which increased rapidly but returned to basal levels by 48 h. In contrast, increased transcription of CCL3, CCL8, and CXCL12, responsible for mononuclear cell migration and chronic inflammation, was sustained. In vitro cell migration assays showed vigorous migration of granulocytes, followed by migration of mononuclear cells. The autologous MLR showed that sAc induced a dose-dependent proliferation of CD4 + T cells and an even stronger proliferation of CD8 + T cells. This effect was inhibited or reduced by pretreatment with mAb to CD11b, MHC II, or MHC II plus CD18. These results indicate that stimulation of bovine PBMC by SAg induces differentiation of monocytes into DC.

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Title: Superantigen-mediated differentiation of bovine monocytes into dendritic cells
Creators: Keun Seok Seo - Departments of
Joo Youn Park - Departments of
William C Davis - Departments of
Lawrence K Fox - Departments of
Mark A McGuire - Departments of
Yong Ho Park - Departments of
Gregory A Bohach - Departments of
Publication Details: Journal of leukocyte biology, Vol.85(4), pp.606-616
Academic Unit: Veterinary Microbiology and Pathology, Department of
Publisher: The Society for Leukocyte Biology
Identifiers: 99900546564301842
Language: English
Resource Type: Journal article