Journal article
Surface envelope glycoprotein is B-lymphocyte immunodominant in sheep naturally infected with ovine progressive pneumonia virus
Clinical and diagnostic laboratory immunology, Vol.12(6), pp.797-800
06/2005
Handle:
https://hdl.handle.net/2376/114608
PMCID: PMC1151977
PMID: 15939757
Abstract
The B-lymphocyte-immunodominant antigen involved in naturally ovine progressive pneumonia virus (OPPV)-infected mature sheep remains unknown. Therefore, the amount of antibody in sera from 10 naturally OPPV-infected sheep was evaluated by immunoprecipitation (IP) of the major viral proteins in [(35)S]methionine/cysteine-labeled OPPV (whole virus) lysate. Using an excess of OPPV proteins in whole-virus lysate, 8 out of 10 sheep had the highest serum antibody IP endpoint titers to the gp135 surface envelope glycoprotein (SU). Also, 2 out of 10 sheep had equivalent serum antibody IP endpoint titers to the transmembrane glycoprotein oligomer (TM90) and SU. Since these data indicate that SU is the immunodominant protein in most mature sheep persistently infected with OPPV, SU-specific diagnostic serological assays can be utilized for OPPV diagnosis.
Metrics
4 Record Views
Details
- Title
- Surface envelope glycoprotein is B-lymphocyte immunodominant in sheep naturally infected with ovine progressive pneumonia virus
- Creators
- Lynn M Herrmann - Animal Disease Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Pullman, Washington 99164-6630, USA. lherrman@vetmed.wsu.eduTravis C McGuireIsidro HötzelGregory S LewisDonald P Knowles
- Publication Details
- Clinical and diagnostic laboratory immunology, Vol.12(6), pp.797-800
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- United States
- Identifiers
- 99900548330301842
- Language
- English
- Resource Type
- Journal article