Journal article
Suspension microarray with dendrimer signal amplification allows direct and high-throughput subtyping of Listeria monocytogenes from genomic DNA
Journal of clinical microbiology, Vol.43(7), pp.3255-3259
07/2005
Handle:
https://hdl.handle.net/2376/113617
PMCID: PMC1169114
PMID: 16000444
Abstract
Listeria monocytogenes is a significant cause of food-borne disease and mortality; therefore, epidemiological investigations of this pathogen require subtyping methods that are rapid, discriminatory, and reproducible. Although conventional microarray subtyping analysis has been shown to be both high resolution and genetically informative, it is still relatively low throughput and technically challenging. Suspension microarray technology eliminates the technical issues associated with planar microarrays and allows high-throughput subtyping of L. monocytogenes strains. In this study, a suspension array assay using dendrimer signal amplification allowed rapid and accurate serovar identification of L. monocytogenes strains using genomic DNA as a target. The ability to subtype genomic DNA without PCR amplification allows probes to be designed for many different regions within the bacterial genome and should allow high-resolution subtyping not possible with multiplex PCR.
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Details
- Title
- Suspension microarray with dendrimer signal amplification allows direct and high-throughput subtyping of Listeria monocytogenes from genomic DNA
- Creators
- Monica K Borucki - USDA-ARS, Animal Disease Research Unit, Pullman, WA 99164-6630, USA. mborucki@vetmed.wsu.eduJames ReynoldsDouglas R CallTodd J WardBrent PageJames Kadushin
- Publication Details
- Journal of clinical microbiology, Vol.43(7), pp.3255-3259
- Academic Unit
- Paul G. Allen School for Global Animal Health
- Publisher
- United States
- Grant note
- N01AI30055 / NIAID NIH HHS
- Identifiers
- 99900547402001842
- Language
- English
- Resource Type
- Journal article