Symbiotic root nodules of the actinorhizal plant Datisca glomerata express Rubisco activase mRNA

P A Okubara; K Pawlowski; T M Murphy; A M Berry

doi:10.1104/pp.120.2.411

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Symbiotic root nodules of the actinorhizal plant Datisca glomerata express Rubisco activase mRNA

Journal article

Open access

Symbiotic root nodules of the actinorhizal plant Datisca glomerata express Rubisco activase mRNA

P A Okubara, K Pawlowski, T M Murphy and A M Berry

Plant physiology (Bethesda), Vol.120(2), pp.411-420

06/1999

DOI: https://doi.org/10.1104/pp.120.2.411

Handle:

https://hdl.handle.net/2376/107858

PMID: 10364392

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Abstract

Symbiosis

Amino Acid Sequence

Gene Expression

Genes, Plant

Introns

RNA, Messenger - genetics

Molecular Sequence Data

Plants - microbiology

DNA Primers - genetics

RNA, Plant - genetics

Plant Roots - microbiology

RNA, Messenger - metabolism

RNA, Plant - metabolism

Actinomycetales - physiology

Plants - enzymology

Sequence Homology, Amino Acid

Plant Proteins - genetics

In Situ Hybridization

Plants - genetics

Base Sequence

Plant Roots - enzymology

DNA, Plant - genetics

N2-fixing symbiotic root nodules of the actinorhizal host Datisca glomerata express Dgrca (D. glomerata Rubisco activase) mRNA, a transcript usually associated with photosynthetic organs or tissues. In northern blots a mature, 1700-nucleotide Dgrca mRNA was detected in green plant organs (leaves, flowers, and developing fruits) and in nodules but was not detected in roots. A second message of 3000 nucleotides was observed only in nodules. Both size classes of transcripts were polyadenylated. The larger transcript was 2- to 5-fold more abundant than the mature mRNA; it was hybridized to an intronic probe, indicating that a stable, incompletely spliced transcript was accumulating. Treatment with light on excised nodules did not alter the relative abundance of the two species. In in situ hybridizations the Dgrca message was expressed intensely in the nuclei of infected cells. The Dgrca transcripts also accumulated at lower levels in uninfected cortical cells adjacent to the periderm and the vascular cylinder. mRNA encoding the large subunit of Rubisco (DgrbcL) was abundant in mature infected cells and in the amyloplast-rich sheath of uninfected cortical cells lying between the infected cells and nodule periderm. The proteins Rubisco activase, Rubisco, and the 33-kD O2-evolving complex subunit did not accumulate to detectable levels, indicating that a functional photosynthetic apparatus was not prevalent in nodule tissue. Signals or factors required for the transcription of Dgrca appeared to be present in nodules, but efficient splicing and translation of the message were not observed in Frankia-infected tissue where transcript accumulation was highest.

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Title: Symbiotic root nodules of the actinorhizal plant Datisca glomerata express Rubisco activase mRNA
Creators: P A Okubara - Department of Environmental Horticulture, University of California, Davis 95616, USA
K Pawlowski
T M Murphy
A M Berry
Publication Details: Plant physiology (Bethesda), Vol.120(2), pp.411-420
Academic Unit: Plant Pathology, Department of
Publisher: United States
Identifiers: 99900547075301842
Language: English
Resource Type: Journal article