Journal article
Synthetic influenza viral double‐stranded RNA induces an acute‐phase response in rabbits
Journal of medical virology, Vol.57(2), pp.198-203
02/1999
Handle:
https://hdl.handle.net/2376/113699
PMID: 9892408
Abstract
Numerous studies have characterized the physiological effects of synthetic, high‐molecular‐weight, homopolymeric, double‐stranded RNA (dsRNA), particularly polyriboinosinic·polyribo‐ cytidylic acid [Carter and De Clercq (1974): Science 186:1172–1178], but limited information exists regarding the physiological effects of dsRNA of viral composition and size. In this report, we determined sleep and fever responses of rabbits to intracerebroventricular injection of different doses of synthetic viral dsRNA (either 108 base pairs or 661 base pairs) derived from the N‐terminal sequence of gene segment 3 of the A/PR/8/34‐H1N1 (PR8) influenza virus. Both the108‐mer and the 661‐mer dsRNAs increased nonrapid eye movement sleep, suppressed rapid eye movement sleep, and induced fever. The 661‐mer dsRNA had more potent somnogenic and pyrogenic effects than the 108‐mer dsRNA on the basis of weight. Neither single‐stranded RNA from the corresponding sequences had significant effects on sleep or brain temperature. These results demonstrate for the first time that low‐molecular‐weight, viral dsRNA has the stability in vivo that is required to induce the fever and sleep changes found in natural viral infections, and the hypothesis is supported that virus‐associated dsRNA may be responsible for initiating the acute‐phase response during viral infections. J. Med. Virol. 57:198–203, 1999. © 1999 Wiley‐Liss, Inc.
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Details
- Title
- Synthetic influenza viral double‐stranded RNA induces an acute‐phase response in rabbits
- Creators
- Jidong FangSebastian BredowPing TaishiJeannine A MajdeJames M Krueger
- Publication Details
- Journal of medical virology, Vol.57(2), pp.198-203
- Academic Unit
- Integrative Physiology and Neuroscience, Department of
- Publisher
- John Wiley & Sons, Inc; New York
- Number of pages
- 6
- Grant note
- Office of Naval Research, Research Opportunities for Program Officers (N00014‐90‐J‐1069) National Institutes of Child Health and Human Development (HD36520)
- Identifiers
- 99900547665701842
- Language
- English
- Resource Type
- Journal article