Journal article
THE CLONING OF A RAT PEPTIDOGLYCAN RECOGNITION PROTEIN (PGRP) AND ITS INDUCTION IN BRAIN BY SLEEP DEPRIVATION
Cytokine (Philadelphia, Pa.), Vol.13(1), pp.8-17
01/2001
Handle:
https://hdl.handle.net/2376/117427
PMID: 11145837
Abstract
Peptidoglycan recognition protein (PGRP) binds to peptidoglycan (PG) or live bacteria and is upregulated by PG. PGRP is a ubiquitous protein involved in innate immunity. Tag7, a novel cytokine, is also induced by bacterial products; tag7 is apoptotic to murine L929 cells in a NF-κB-independent manner. Both of these genes are expressed in brain, lymphatic and haematopoietic tissues. We provide evidence that murine PGRP and tag7 encode identical transcripts and have structural relationships to lysozymes. Further, we have cloned the cDNA of rat PGRP and analyzed its expression in brains of sleep-deprived and control rats. The mRNA levels of PGRP/tag7 were measured by RT-PCR and compared to the housekeeping gene porphobilinogen deaminase (PBD). PGRP was constitutively expressed in rat brain. PGRP mRNA was increased by 43% and 17% in the brainstem and hypothalamus, respectively, in sleep-deprived rats compared to controls. The upregulation of PGRP expression by sleep deprivation suggests a role for PGRP in a homeostatic regulation of sleep.
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Details
- Title
- THE CLONING OF A RAT PEPTIDOGLYCAN RECOGNITION PROTEIN (PGRP) AND ITS INDUCTION IN BRAIN BY SLEEP DEPRIVATION
- Creators
- Abdur Rehman - Department of VCAPP, Washington State University, Pullman, WA, 99164, USAPing Taishi - Department of VCAPP, Washington State University, Pullman, WA, 99164, USAJidong Fang - Department of VCAPP, Washington State University, Pullman, WA, 99164, USAJeannine A Majde - Office of Naval Research, Arlington, VA, 22217, USAJames M Krueger - Department of VCAPP, Washington State University, Pullman, WA, 99164, USA
- Publication Details
- Cytokine (Philadelphia, Pa.), Vol.13(1), pp.8-17
- Academic Unit
- Integrative Physiology and Neuroscience, Department of
- Publisher
- Elsevier Ltd
- Identifiers
- 99900548249001842
- Language
- English
- Resource Type
- Journal article