Journal article
TNFα siRNA reduces brain TNF and EEG delta wave activity in rats
Brain research, Vol.1156(1), pp.125-132
2007
Handle:
https://hdl.handle.net/2376/105995
PMCID: PMC2041959
PMID: 17531209
Abstract
Tumor necrosis factor alpha (TNFα) is a pleiotropic cytokine with several CNS physiological and pathophysiological actions including sleep, memory, thermal and appetite regulation. Short interfering RNAs (siRNA) targeting TNFα were incubated with cortical cell cultures and microinjected into the primary somatosensory cortex (SSctx) of rats. The TNFα siRNA treatment specifically reduced TNFα mRNA by 45%
in vitro without affecting interleukin-6 or gluR1–4 mRNA levels.
In vivo the TNFα siRNAα reduced TNFα mRNA, interleukin-6 mRNA and gluR1 mRNA levels compared to treatment with a scrambled control siRNA. After
in vivo microinjection, the density of TNFα-immunoreactive cells in layer V of the SSctx was also reduced. Electroencephalogram (EEG) delta wave power was decreased on days 2 and 3 on the side of the brain that received the TNFα siRNA microinjection relative to the side receiving the control siRNA. These findings support the hypothesis that TNFα siRNA attenuates TNFα mRNA and TNFα protein in the rat cortex and that those reductions reduce cortical EEG delta power. Results also are consistent with the notion that TNFα is involved in CNS physiology including sleep regulation.
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Details
- Title
- TNFα siRNA reduces brain TNF and EEG delta wave activity in rats
- Creators
- Ping TaishiLynn ChurchillMingxiang WangDaniel KayChristopher J DavisXin GuanAlok DeTadanobu YasudaFan LiaoJames M Krueger
- Publication Details
- Brain research, Vol.1156(1), pp.125-132
- Academic Unit
- Biomedical Sciences, Department of; Integrative Physiology and Neuroscience, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900546942501842
- Language
- English
- Resource Type
- Journal article