Journal article
Tandem catalysis driven by enzymes directed hybrid nanoflowers for on-site ultrasensitive detection of organophosphorus pesticide
Biosensors & bioelectronics, Vol.141, p.111473
09/15/2019
PMID: 31272060
Abstract
Accurate analysis of organophosphate pesticides (OPs) with portable devices remain an elusive goal that have received widespread investigative attention in the areas of environmental contamination and disease prevention. Herein, using all-in-one enzyme-inorganic hybrid nanoflowers (ACC-HNFs) to fabricate high-performance artificial enzyme cascade system, we established a sensitive and affordable lab-on-paper biosensor. This biosensor incorporated disposable screen-printed carbon electrode (SPCE) and colorimetric test strips, which enabled the dual-modal readout (electrochemical and colorimetric signal) for on-site monitoring of OPs, achieving an “on-demand” tuning of the detection performance. Using paraoxon as a model analyte, the ACC-HNFs-based lab-on-paper platform could reach a limit of detection down to the femtogram/mL level (6 fg mL−1). Meticulous design of ACC-HNFs provided a versatile approach for constructing artificial enzyme as a recognizer and amplifier to fill the gap in constructing robust artificial enzyme systems which can be used for on-site contamination monitoring and biological diagnosis.
•A dual-modal readout biosensor was proposed to detect pesticide.•The HNFs showed high catalytic activity by integrating nanozyme and natural enzyme.•High performance: A limit of detection down to the femtogram/mL level (6 fg mL−1).•Screen-printed electrode combined with test strips for on-site monitoring of OPs.
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Details
- Title
- Tandem catalysis driven by enzymes directed hybrid nanoflowers for on-site ultrasensitive detection of organophosphorus pesticide
- Creators
- Rui Jin - Jilin UniversityDeshuai Kong - Jilin UniversityXu Zhao - Jilin UniversityHongxia Li - Jilin UniversityXu Yan - Jilin UniversityFangmeng Liu - Jilin UniversityPeng Sun - Jilin UniversityDan Du - Washington State UniversityYuehe Lin - Washington State UniversityGeyu Lu - Jilin University
- Publication Details
- Biosensors & bioelectronics, Vol.141, p.111473
- Academic Unit
- School of Mechanical and Materials Engineering
- Publisher
- Elsevier B.V
- Identifiers
- 99901227843301842
- Language
- English
- Resource Type
- Journal article