Temperature response of Rubisco kinetics in Arabidopsis thaliana: thermal breakpoints and implications for reaction mechanisms

Ryan A Boyd; Amanda P Cavanagh; David S Kubien; Asaph B Cousins

doi:10.1093/jxb/ery355

Back

Temperature response of Rubisco kinetics in Arabidopsis thaliana: thermal breakpoints and implications for reaction mechanisms

Journal article

Open access

Peer reviewed

Temperature response of Rubisco kinetics in Arabidopsis thaliana: thermal breakpoints and implications for reaction mechanisms

Ryan A Boyd, Amanda P Cavanagh, David S Kubien and Asaph B Cousins

Journal of experimental botany, Vol.70(1), pp.231-242

01/01/2019

DOI: https://doi.org/10.1093/jxb/ery355

Handle:

https://hdl.handle.net/2376/101214

PMCID: PMC6305185

PMID: 30403800

Files and links (1)

url

https://doi.org/10.1093/jxb/ery355View

Published (Version of record) Open

Abstract

Arabidopsis - physiology

Temperature

Ribulose-Bisphosphate Carboxylase - physiology

Botany - methods

Photosynthesis - physiology

Kinetics

Mass Spectrometry - methods

Enhancement of Rubisco kinetics could improve photosynthetic efficiency, ultimately resulting in increased crop yield. However, imprecise knowledge of the reaction mechanism and the individual rate constants limits our ability to optimize the enzyme. Membrane inlet mass spectrometry (MIMS) may offer benefits over traditional methods for determining individual rate constants of the Rubisco reaction mechanism, as it can directly monitor concentration changes in CO2, O2, and their isotopologs during assays. However, a direct comparison of MIMS with the traditional radiolabel method of determining Rubisco kinetic parameters has not been made. Here, the temperature responses of Rubisco kinetic parameters from Arabidopsis thaliana were measured using radiolabel and MIMS methods. The two methods provided comparable parameters above 25 °C, but temperature responses deviated at low temperature as MIMS-derived catalytic rates of carboxylation, oxygenation, and CO2/O2 specificity showed thermal breakpoints. Here, we discuss the variability and uncertainty surrounding breakpoints in the Rubisco temperature response and the relevance of individual rate constants of the reaction mechanisms to potential breakpoints.

Metrics

5 Record Views

See more details

Details

Title: Temperature response of Rubisco kinetics in Arabidopsis thaliana: thermal breakpoints and implications for reaction mechanisms
Creators: Ryan A Boyd - School of Biological Sciences, Molecular Plant Sciences, Washington State University, Pullman, WA, USA
Amanda P Cavanagh - Department of Biology, University of New Brunswick, Fredericton, NB, Canada
David S Kubien - Department of Biology, University of New Brunswick, Fredericton, NB, Canada
Asaph B Cousins - School of Biological Sciences, Molecular Plant Sciences, Washington State University, Pullman, WA, USA
Publication Details: Journal of experimental botany, Vol.70(1), pp.231-242
Academic Unit: Biological Sciences, School of
Publisher: England
Identifiers: 99900546551901842
Language: English
Resource Type: Journal article