Temporal characterisation of the organ-specific Rhipicephalus microplus transcriptional response to Anaplasma marginale infection

Ricardo F Mercado-Curiel; Guy H Palmer; Felix D Guerrero; Kelly A Brayton

doi:10.1016/j.ijpara.2011.03.003

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Temporal characterisation of the organ-specific Rhipicephalus microplus transcriptional response to Anaplasma marginale infection

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Temporal characterisation of the organ-specific Rhipicephalus microplus transcriptional response to Anaplasma marginale infection

Ricardo F Mercado-Curiel, Guy H Palmer, Felix D Guerrero and Kelly A Brayton

International journal for parasitology, Vol.41(8), pp.851-860

07/2011

DOI: https://doi.org/10.1016/j.ijpara.2011.03.003

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https://hdl.handle.net/2376/103835

PMID: 21514300

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Abstract

Host-Pathogen Interactions

Animals

Rhipicephalus - genetics

Anaplasma marginale - pathogenicity

Gastrointestinal Tract - microbiology

Male

Gene Expression Profiling

Salivary Glands - microbiology

Rhipicephalus - microbiology

Arthropods transmit important infectious diseases of humans and animals. Importantly, replication and the development of pathogen infectivity are tightly linked to vector feeding on the mammalian host; thus analysis of the transcriptomes of both vector and pathogen during feeding is fundamental to understanding transmission. Using Anaplasma marginale infection of Rhipicephalus microplus as the experimental model, we tested three hypotheses exploring the temporal and organ-specific nature of the tick midgut and salivary gland transcriptomes during feeding and in response to infection. Numerous R. microplus genes were regulated in response to feeding and were differentially regulated between the midgut and salivary gland; additionally, there was a progression in regulated gene expression in the salivary gland over time. In contrast, relatively few tick genes were specifically regulated in response to A. marginale infection and these genes were predominantly annotated as hypothetical or were of unknown function. Notable among the genes with informative annotation was that several ribosomal proteins were down-regulated, suggesting that there may be a corresponding decrease in translation. The hypotheses that R. microplus midgut and salivary gland genes are differentially regulated and that the salivary gland transcriptome is dynamic over time were accepted. This is consistent with, and important for understanding the roles of, the two organs, the midgut serving as an initial site of uptake and replication while the salivary gland serves as the final site of replication and secretion. The nominal effect of A. marginale on the tick transcriptome in terms of numbers of regulated genes and fold of regulation supports the view that the vector-pathogen relationship is well established with minimal deleterious effect on the tick. The small set of predominantly hypothetical genes regulated by infection suggests that A. marginale is affecting a novel set of tick genes and may provide new opportunities for blocking transmission from the tick.

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Title: Temporal characterisation of the organ-specific Rhipicephalus microplus transcriptional response to Anaplasma marginale infection
Creators: Ricardo F Mercado-Curiel - Department of Veterinary Microbiology and Pathology and Paul G Allen School for Global Animal Health, Washington State University, Pullman, WA 99164-7040, USA
Guy H Palmer
Felix D Guerrero
Kelly A Brayton
Publication Details: International journal for parasitology, Vol.41(8), pp.851-860
Academic Unit: Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
Publisher: England
Grant note: R37 AI044005 / NIAID NIH HHS GR075800M / Wellcome Trust
Identifiers: 99900546748701842
Language: English
Resource Type: Journal article