Testicular peritubular cells secrete a protein under androgen control that modulates Sertoli cell functions

M K Skinner; I B Fritz

doi:10.1073/pnas.82.1.114

Back

Testicular peritubular cells secrete a protein under androgen control that modulates Sertoli cell functions

Journal article

Open access

Testicular peritubular cells secrete a protein under androgen control that modulates Sertoli cell functions

M K Skinner and I B Fritz

Proceedings of the National Academy of Sciences - PNAS, Vol.82(1), pp.114-118

01/1985

DOI: https://doi.org/10.1073/pnas.82.1.114

Handle:

https://hdl.handle.net/2376/106176

PMID: 3855533

Files and links (1)

pdf

testicular-peritubular-cells1.15 MBDownload View

Open Access

Abstract

Proteins - physiology

Testis - metabolism

Androgens - physiology

Molecular Weight

Androgen-Binding Protein - biosynthesis

Testis - cytology

Carrier Proteins - biosynthesis

Cells, Cultured

Testosterone - pharmacology

Testis - physiology

Sertoli Cells - physiology

Animals

Culture Media

Estradiol - pharmacology

Transferrin - biosynthesis

Reproduction

Peritubular cells of the seminiferous tubule synthesize component(s) that stimulate Sertoli cells in culture to increase the production of androgen-binding protein and testicular transferrin. The active peritubular cell component(s) are trypsin-sensitive, heat-sensitive, acid-stable molecule(s) having a molecular weight between 50,000 and 100,000. These specific factors(s) are referred to as P Mod-S to designate protein(s), produced by peritubular cells (P), that modulate the functions of Sertoli cells (S). The degree of stimulation by P Mod-S is comparable to that obtained by maximal hormonal stimulation of the synthesis of ABP and transferrin by Sertoli cells. Levels of P Mod-S secreted into the medium by primary cultures of peritubular cells are increased in the presence of testosterone. Comparable concentrations of 17 beta-estradiol do not stimulate peritubular cells to synthesize P Mod-S. Data are interpreted to indicate that androgens act on testicular peritubular cells to increase the formation of P Mod-S and that P Mod-S may modulate the properties of adjacent Sertoli cells. Findings are discussed in relation to the nature of mesenchymal-epithelial cell interactions in the seminiferous tubule and to the possible role of P Mod-S as a mediator of androgen actions of Sertoli cells.

Metrics

1 File views/ downloads

4 Record Views

See more details

Details

Title: Testicular peritubular cells secrete a protein under androgen control that modulates Sertoli cell functions
Creators: M K Skinner
I B Fritz
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.82(1), pp.114-118
Academic Unit: Biological Sciences, School of
Publisher: United States
Identifiers: 99900546955801842
Language: English
Resource Type: Journal article