The ABC transporter PXA1 and peroxisomal beta-oxidation are vital for metabolism in mature leaves of Arabidopsis during extended darkness

Hans-Henning Kunz; Michael Scharnewski; Kirstin Feussner; Ivo Feussner; Ulf-Ingo Flügge; Martin Fulda; Markus Gierth

doi:10.1105/tpc.108.064857

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The ABC transporter PXA1 and peroxisomal beta-oxidation are vital for metabolism in mature leaves of Arabidopsis during extended darkness

Journal article

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Peer reviewed

The ABC transporter PXA1 and peroxisomal beta-oxidation are vital for metabolism in mature leaves of Arabidopsis during extended darkness

Hans-Henning Kunz, Michael Scharnewski, Kirstin Feussner, Ivo Feussner, Ulf-Ingo Flügge, Martin Fulda and Markus Gierth

The Plant cell, Vol.21(9), pp.2733-2749

09/2009

DOI: https://doi.org/10.1105/tpc.108.064857

Handle:

https://hdl.handle.net/2376/104520

PMCID: PMC2768912

PMID: 19794119

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Abstract

Darkness

Chlorophyll - analogs & derivatives

Starch - metabolism

Arabidopsis Proteins - metabolism

ATP-Binding Cassette Transporters - genetics

Potassium Channels, Voltage-Gated - metabolism

Adenosine Triphosphate - metabolism

Cloning, Molecular

ATP-Binding Cassette Transporters - metabolism

Potassium Channels, Voltage-Gated - genetics

Peroxisomes - genetics

Arabidopsis Proteins - genetics

Oxidation-Reduction

Chlorophyll - metabolism

Peroxisomes - metabolism

Arabidopsis - metabolism

Photosystem II Protein Complex - metabolism

Arabidopsis - genetics

DNA, Bacterial - genetics

Plant Leaves - genetics

Plant Leaves - metabolism

alpha-Linolenic Acid - metabolism

Mutagenesis, Insertional

Mutation

Photosynthesis

Fatty acid beta-oxidation is essential for seedling establishment of oilseed plants, but little is known about its role in leaf metabolism of adult plants. Arabidopsis thaliana plants with loss-of-function mutations in the peroxisomal ABC-transporter1 (PXA1) or the core beta-oxidation enzyme keto-acyl-thiolase 2 (KAT2) have impaired peroxisomal beta-oxidation. pxa1 and kat2 plants developed severe leaf necrosis, bleached rapidly when returned to light, and died after extended dark treatment, whereas the wild type was unaffected. Dark-treated pxa1 plants showed a decrease in photosystem II efficiency early on and accumulation of free fatty acids, mostly alpha-linolenic acid [18:3(n-3)] and pheophorbide a, a phototoxic chlorophyll catabolite causing the rapid bleaching. Isolated wild-type and pxa1 chloroplasts challenged with comparable alpha-linolenic acid concentrations both showed an 80% reduction in photosynthetic electron transport, whereas intact pxa1 plants were more susceptible to the toxic effects of alpha-linolenic acid than the wild type. Furthermore, starch-free mutants with impaired PXA1 function showed the phenotype more quickly, indicating a link between energy metabolism and beta-oxidation. We conclude that the accumulation of free polyunsaturated fatty acids causes membrane damage in pxa1 and kat2 plants and propose a model in which fatty acid respiration via peroxisomal beta-oxidation plays a major role in dark-treated plants after depletion of starch reserves.

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Title: The ABC transporter PXA1 and peroxisomal beta-oxidation are vital for metabolism in mature leaves of Arabidopsis during extended darkness
Creators: Hans-Henning Kunz - Department of Botany II, University of Cologne, Koeln, Germany
Michael Scharnewski
Kirstin Feussner
Ivo Feussner
Ulf-Ingo Flügge
Martin Fulda
Markus Gierth
Publication Details: The Plant cell, Vol.21(9), pp.2733-2749
Academic Unit: Biological Sciences, School of
Publisher: United States
Identifiers: 99900546898301842
Language: English
Resource Type: Journal article