Journal article
The Anaplasma marginale msp5 gene encodes a 19-kilodalton protein conserved in all recognized Anaplasma species
Infection and immunity, Vol.60(12), pp.5139-5144
12/1992
Handle:
https://hdl.handle.net/2376/106386
PMCID: PMC258289
PMID: 1280624
Abstract
Immunization with Anaplasma marginale outer membranes induced immunity against clinical disease which correlated with antibody titer to outer membrane proteins, including a 19-kDa protein (N. Tebele, T. C. McGuire, and G. H. Palmer, Infect. Immun. 59:3199-3204, 1991). This 19-kDa protein, designated major surface protein 5 (MSP-5), was encoded by a single-copy 633-bp gene. The molecular mass of MSP-5, defined in immunoblots by binding to monoclonal antibody ANAF16C1, was conserved among all recognized species of Anaplasma: A. marginale, A. centrale, and A. ovis. Recombinant MSP-5, which absorbed the antibody reactivity of bovine immune serum to native MSP-5, was recognized by anti-A. marginale and anti-A. centrale immune sera in a competitive inhibition assay with monoclonal antibody ANAF16C1. The presence of antibody to the epitope defined by monoclonal antibody ANAF16C1 in all postinfection sera tested indicates that this epitope is a potential diagnostic antigen for use in identifying persistently infected cattle.
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Details
- Title
- The Anaplasma marginale msp5 gene encodes a 19-kilodalton protein conserved in all recognized Anaplasma species
- Creators
- E S Visser - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040T C McGuire - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040G H Palmer - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040W C Davis - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040V Shkap - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040E Pipano - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040D P Knowles - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040
- Publication Details
- Infection and immunity, Vol.60(12), pp.5139-5144
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Identifiers
- 99900546709301842
- Language
- English
- Resource Type
- Journal article