Journal article
The Arabidopsis SLEEPY1 Gene Encodes a Putative F-Box Subunit of an SCF E3 Ubiquitin Ligase
The Plant cell, Vol.15(5), pp.1120-1130
05/2003
Handle:
https://hdl.handle.net/2376/108371
PMCID: PMC153720
PMID: 12724538
Abstract
The Arabidopsis
SLY1
(
SLEEPY1
) gene positively regulates gibberellin (GA) signaling. Positional cloning of
SLY1
revealed that it encodes a putative F-box protein. This result suggests that SLY1 is the F-box subunit of an SCF E3 ubiquitin ligase that regulates GA responses. The DELLA domain protein RGA (repressor of
ga1-3
) is a repressor of GA response that appears to undergo GA-stimulated protein degradation. RGA is a potential substrate of SLY1, because
sly1
mutations cause a significant increase in RGA protein accumulation even after GA treatment. This result suggests SCF
SLY1
-targeted degradation of RGA through the 26S proteasome pathway. Further support for this model is provided by the observation that an
rga
null allele partially suppresses the
sly1-10
mutant phenotype. The predicted SLY1 amino acid sequence is highly conserved among plants, indicating a key role in GA response.
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Details
- Title
- The Arabidopsis SLEEPY1 Gene Encodes a Putative F-Box Subunit of an SCF E3 Ubiquitin Ligase
- Creators
- Karen M McGinnis - United States Department of Agriculture–Agricultural Research Service, Washington State University, Pullman, Washington 99164-6420Stephen G Thomas - Department of Biology, Duke University, Durham, North Carolina 27708-1000Jonathan D Soule - United States Department of Agriculture–Agricultural Research Service, Washington State University, Pullman, Washington 99164-6420Lucia C Strader - Plant Physiology and Biochemistry, Washington State University, Pullman, Washington 99164-6420Janice M Zale - United States Department of Agriculture–Agricultural Research Service, Washington State University, Pullman, Washington 99164-6420Tai-ping Sun - Department of Biology, Duke University, Durham, North Carolina 27708-1000Camille M Steber - United States Department of Agriculture–Agricultural Research Service, Washington State University, Pullman, Washington 99164-6420
- Publication Details
- The Plant cell, Vol.15(5), pp.1120-1130
- Academic Unit
- Crop and Soil Sciences, Department of
- Publisher
- American Society of Plant Biologists
- Identifiers
- 99900547520601842
- Language
- English
- Resource Type
- Journal article