The Babesia bovis merozoite surface antigen 1 hypervariable region induces surface-reactive antibodies that block merozoite invasion

Tanya LeRoith; Shawn J Berens; Kelly A Brayton; Stephen A Hines; Wendy C Brown; Junzo Norimine; Terry F McElwain

doi:10.1128/IAI.00032-06

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The Babesia bovis merozoite surface antigen 1 hypervariable region induces surface-reactive antibodies that block merozoite invasion

Journal article

Open access

Peer reviewed

The Babesia bovis merozoite surface antigen 1 hypervariable region induces surface-reactive antibodies that block merozoite invasion

Tanya LeRoith, Shawn J Berens, Kelly A Brayton, Stephen A Hines, Wendy C Brown, Junzo Norimine and Terry F McElwain

Infection and immunity, Vol.74(6), pp.3663-3667

06/2006

DOI: https://doi.org/10.1128/IAI.00032-06

Handle:

https://hdl.handle.net/2376/112130

PMCID: PMC1479293

PMID: 16714599

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https://doi.org/10.1128/IAI.00032-06View

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Abstract

Amino Acid Sequence

Merozoite Surface Protein 1 - chemistry

Animals

Babesia bovis - immunology

Antibodies, Protozoan - immunology

Merozoite Surface Protein 1 - immunology

Molecular Sequence Data

Antibodies, Monoclonal - immunology

A hypervariable region (HVR) previously identified in the carboxy-terminal one-third of the Babesia bovis variable merozoite surface antigen family was more extensively analyzed in merozoite surface antigen 1 (MSA-1) from 16 strains and isolates. The MSA-1 HVR is proline rich and contains three semiconserved motifs nearly identical to those described for the related family member MSA-2. Two MSA-1-specific monoclonal antibodies previously shown to be reactive with the merozoite surface bound to a recombinant construct encoding the HVR, indicating that the HVR is surface exposed and accessible to antibody binding. Importantly, these surface-reactive, HVR-specific monoclonal antibodies were capable of inhibiting merozoite infectivity of the host erythrocyte in vivo. The results indicate that the MSA-1 HVR is involved in erythrocyte invasion and suggest that selection of MSA-1 variants may be driven by invasion-blocking antibodies.

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Details

Title: The Babesia bovis merozoite surface antigen 1 hypervariable region induces surface-reactive antibodies that block merozoite invasion
Creators: Tanya LeRoith - Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040, USA. tleroith@vt.edu
Shawn J Berens
Kelly A Brayton
Stephen A Hines
Wendy C Brown
Junzo Norimine
Terry F McElwain
Publication Details: Infection and immunity, Vol.74(6), pp.3663-3667
Academic Unit: Veterinary Medicine, College of; Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
Publisher: United States
Grant note: 1 K08 AI151391 / NIAID NIH HHS
Identifiers: 99900547509801842
Language: English
Resource Type: Journal article