Journal article
The C-Terminal Variable Region Specifies the Dynamic Properties of Arabidopsis Microtubule-Associated Protein MAP65 Isotypes[C][W]
The Plant cell, Vol.20(12), pp.3346-3358
12/2008
Handle:
https://hdl.handle.net/2376/102651
PMCID: PMC2630438
PMID: 19060108
Abstract
The microtubule-associated protein, MAP65, is a member of a family of divergent microtubule-associated proteins from different organisms generally involved in maintaining the integrity of the central spindle in mitosis. The dicotyledon
Arabidopsis thaliana
and the monocotyledon rice (
Oryza sativa
) genomes contain 9 and 11 MAP65 genes, respectively. In this work, we show that the majority of these proteins fall into five phylogenetic clades, with the greatest variation between clades being in the C-terminal random coil domain. At least one
Arabidopsis
and one rice isotype is within each clade, indicating a functional specification for the C terminus. In At MAP65-1, the C-terminal domain is a microtubule binding region (MTB2) harboring the phosphorylation sites that control its activity. The At MAP65 isotypes show differential localization to microtubule arrays and promote microtubule polymerization with variable efficiency in a MTB2-dependent manner. In vivo studies demonstrate that the dynamics of the association and dissociation of different MAP65 isotypes with microtubules can vary up to 10-fold and that this correlates with their ability to promote microtubule polymerization. Our data demonstrate that the C-terminal variable region, MTB2, determines the dynamic properties of individual isotypes and suggest that slower turnover is conditional for more efficient microtubule polymerization.
Metrics
7 Record Views
Details
- Title
- The C-Terminal Variable Region Specifies the Dynamic Properties of Arabidopsis Microtubule-Associated Protein MAP65 Isotypes[C][W]
- Creators
- Andrei P Smertenko - Integrative Cell Biology Laboratory, School of Biological and Biomedical Sciences, University of Durham, Durham DH1 3LE, United KingdomDespina Kaloriti - Integrative Cell Biology Laboratory, School of Biological and Biomedical Sciences, University of Durham, Durham DH1 3LE, United KingdomHsin-Yu Chang - Integrative Cell Biology Laboratory, School of Biological and Biomedical Sciences, University of Durham, Durham DH1 3LE, United KingdomJindriska Fiserova - Integrative Cell Biology Laboratory, School of Biological and Biomedical Sciences, University of Durham, Durham DH1 3LE, United KingdomZdenek Opatrny - Department of Plant Physiology, Charles University, Faculty of Science, 128 44 Prague 2,Czech RepublicPatrick J Hussey - Integrative Cell Biology Laboratory, School of Biological and Biomedical Sciences, University of Durham, Durham DH1 3LE, United Kingdom
- Publication Details
- The Plant cell, Vol.20(12), pp.3346-3358
- Academic Unit
- Biological Chemistry, Institute of
- Publisher
- American Society of Plant Biologists
- Identifiers
- 99900546503201842
- Language
- English
- Resource Type
- Journal article