Journal article
The Calcium-Saturated cTnI/cTnC Complex: Structure of the Inhibitory Region of cTnI
Biophysical journal, Vol.84(2), pp.1057-1064
02/2003
Handle:
https://hdl.handle.net/2376/104566
PMCID: PMC1302683
PMID: 12547787
Abstract
The contiguous inhibitory and regulatory regions of troponin I in the heterotrimeric troponin complex play a critical role in Ca
2+
activation of striated muscle. Knowledge of the structure of this critical region within the complex will enhance efforts toward understanding regulatory mechanisms. Toward this goal, we have used simulated annealing to study the structure of the inhibitory and regulatory regions of cardiac muscle troponin I in the calcium-saturated complex formed between cardiac troponin C and cardiac troponin I. We have incorporated distances determined experimentally by Förster resonance energy transfer in the full-length complex, rather than using peptides derived from cTnI. For these models, we assume a helix-loop-helix conformation for the inhibitory region. We have found several structures that satisfy the experimental constraints fairly well. Although it is not possible to eliminate any of these models at this time, future studies with additional experimental restraints will yield insights on the mechanisms of calcium regulation in cardiac muscle.
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Details
- Title
- The Calcium-Saturated cTnI/cTnC Complex: Structure of the Inhibitory Region of cTnI
- Creators
- Christopher Sheldahl - Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, Alabama 35294 USAJun Xing - Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, Alabama 35294 USAWen-Ji Dong - Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, Alabama 35294 USAStephen C Harvey - Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, Alabama 35294 USAHerbert C Cheung - Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, Alabama 35294 USA
- Publication Details
- Biophysical journal, Vol.84(2), pp.1057-1064
- Academic Unit
- Chemical Engineering and Bioengineering, School of
- Publisher
- Biophysical Society
- Identifiers
- 99900546506701842
- Language
- English
- Resource Type
- Journal article