Journal article
The Campylobacter jejuni Response Regulator, CbrR, Modulates Sodium Deoxycholate Resistance and Chicken Colonization
Journal of bacteriology, Vol.187(11), pp.3662-3670
06/2005
Handle:
https://hdl.handle.net/2376/115066
PMCID: PMC1112060
PMID: 15901688
Abstract
Two-component regulatory systems play a major role in the physiological response of bacteria to environmental stimuli. Such systems are composed of a sensor histidine kinase and a response regulator whose ultimate function is to affect the expression of target genes. Response regulator mutants of
Campylobacter jejuni
strain F38011 were screened for sensitivity to sodium deoxycholate. A mutation in Cj0643, which encodes a response regulator with no obvious cognate histidine kinase, resulted in an absence of growth on plates containing a subinhibitory concentration of sodium deoxcholate (1%, wt/vol). In broth cultures containing 0.05% (wt/vol) sodium deoxycholate, growth of the mutant was significantly inhibited compared to growth of the
C. jejuni
F38011 wild-type strain. Complementation of the
C. jejuni cbrR
mutant in
trans
restored growth in both broth and plate cultures supplemented with sodium deoxycholate. Based on the phenotype displayed by its mutation, we designated the gene corresponding to Cj0643 as
cbrR
(
Campylobacter b
ile
r
esistance
r
egulator). While the MICs of a variety of bile salts and other detergents for the
C. jejuni cbrR
mutant were lower, no difference was noted in its sensitivity to antibiotics or osmolarity. Finally, chicken colonization studies demonstrated that the
C. jejuni cbrR
mutant had a reduced ability to colonize compared to the wild-type strain. These data support previous findings that bile resistance contributes to colonization of chickens and establish that the response regulator, CbrR, modulates resistance to bile salts in
C. jejuni
.
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Details
- Title
- The Campylobacter jejuni Response Regulator, CbrR, Modulates Sodium Deoxycholate Resistance and Chicken Colonization
- Creators
- Brian H Raphael - School of Molecular Biosciences, Washington State University, Pullman, Washington 99164Sonia Pereira - School of Molecular Biosciences, Washington State University, Pullman, Washington 99164Gary A Flom - School of Molecular Biosciences, Washington State University, Pullman, Washington 99164Qijing Zhang - School of Molecular Biosciences, Washington State University, Pullman, Washington 99164Julian M Ketley - School of Molecular Biosciences, Washington State University, Pullman, Washington 99164Michael E Konkel - School of Molecular Biosciences, Washington State University, Pullman, Washington 99164
- Publication Details
- Journal of bacteriology, Vol.187(11), pp.3662-3670
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- American Society for Microbiology
- Identifiers
- 99900548178701842
- Language
- English
- Resource Type
- Journal article