Journal article
The Caspase-Related Protease Separase (EXTRA SPINDLE POLES) Regulates Cell Polarity and Cytokinesis in Arabidopsis[C][W]
The Plant cell, Vol.25(6), pp.2171-2186
06/2013
Handle:
https://hdl.handle.net/2376/108124
PMCID: PMC3723619
PMID: 23898031
Abstract
Separase is responsible for segregation of daughter chromatids during cell division in all eukaryotes. Here it is reported that in addition to regulating chromatid segregation, the plant homolog of separase regulates vesicle trafficking essential for the cytokinesis and establishment of cell polarity during tissue and organ patterning.
Vesicle trafficking plays an important role in cell division, establishment of cell polarity, and translation of environmental cues to developmental responses. However, the molecular mechanisms regulating vesicle trafficking remain poorly understood. Here, we report that the evolutionarily conserved caspase-related protease separase (EXTRA SPINDLE POLES [ESP]) is required for the establishment of cell polarity and cytokinesis in
Arabidopsis
thaliana
. At the cellular level, separase colocalizes with microtubules and RabA2a (for RAS GENES FROM RAT BRAINA2a) GTPase-positive structures. Separase facilitates polar targeting of the auxin efflux carrier PIN-FORMED2 (PIN2) to the rootward side of the root cortex cells. Plants with the
radially swollen4
(
rsw4
) allele with compromised separase activity, in addition to mitotic failure, display isotropic cell growth, perturbation of auxin gradient formation, slower gravitropic response in roots, and cytokinetic failure. Measurements of the dynamics of vesicle markers on the cell plate revealed an overall reduction of the delivery rates of KNOLLE and RabA2a GTPase in separase-deficient roots. Furthermore, dissociation of the clathrin light chain, a protein that plays major role in the formation of coated vesicles, was slower in
rsw4
than in the control. Our results demonstrate that separase is a key regulator of vesicle trafficking, which is indispensable for cytokinesis and the establishment of cell polarity.
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Details
- Title
- The Caspase-Related Protease Separase (EXTRA SPINDLE POLES) Regulates Cell Polarity and Cytokinesis in Arabidopsis[C][W]
- Creators
- Panagiotis N Moschou - Department of Plant Biology and Forest Genetics, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, SE-75007 Uppsala, SwedenAndrei P Smertenko - The Integrative Cell Biology Laboratory, School of Biological and Biomedical Sciences, University of Durham, DH1 3LE Durham, United KingdomElena A Minina - Department of Plant Biology and Forest Genetics, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, SE-75007 Uppsala, SwedenKazutake Fukada - Department of Plant Biology and Forest Genetics, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, SE-75007 Uppsala, SwedenEugene I Savenkov - Department of Plant Biology and Forest Genetics, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, SE-75007 Uppsala, SwedenStephanie Robert - Department of Forest Genetics and Plant Physiology, Umeå Plant Science Centre, Swedish University of Agricultural Sciences, 901 83 Umea, SwedenPatrick J Hussey - The Integrative Cell Biology Laboratory, School of Biological and Biomedical Sciences, University of Durham, DH1 3LE Durham, United KingdomPeter V Bozhkov - Department of Plant Biology and Forest Genetics, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, SE-75007 Uppsala, Sweden
- Publication Details
- The Plant cell, Vol.25(6), pp.2171-2186
- Academic Unit
- Biological Chemistry, Institute of
- Publisher
- American Society of Plant Biologists
- Identifiers
- 99900547139201842
- Language
- English
- Resource Type
- Journal article