Journal article
The Development of Fluorescent Probes for Visualizing Intracellular Hydrogen Polysulfides
Angewandte Chemie (International ed.), Vol.54(47), pp.13961-14171
11/16/2015
Handle:
https://hdl.handle.net/2376/115143
PMID: 26381762
Abstract
Endogenous hydrogen polysulfides (H2Sn; n>1) have been recognized as important regulators in sulfur-related redox biology. H2Sn can activate tumor suppressors, ion channels, and transcription factors with higher potency than H2S. Although H2Sn are drawing increasing attention, their exact mechanisms of action are still poorly understood. A major hurdle in this field is the lack of reliable and convenient methods for H2Sn detection. Herein we report a H2Sn-mediated benzodithiolone formation under mild conditions. This method takes advantage of the unique dual reactivity of H2Sn as both a nucleophile and an electrophile. Based on this reaction, three fluorescent probes (PSP-1, PSP-2, and PSP-3) were synthesized and evaluated. Among the probes prepared, PSP-3 showed a desirable off/on fluorescence response to H2Sn and high specificity. The probe was successfully applied in visualizing intracellular H2Sn.
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Details
- Title
- The Development of Fluorescent Probes for Visualizing Intracellular Hydrogen Polysulfides
- Creators
- Wei Chen - Department of Chemistry, Washington State University, Pullman, WA 99164 (USA)Ethan W Rosser - Department of Chemistry, Washington State University, Pullman, WA 99164 (USA)Tetsuro Matsunaga - Department of Environmental Health Sciences and Molecular Toxicology, Tohoku University Graduate School of Medicine, Sendai, 980-8575 (Japan)Armando Pacheco - Department of Chemistry, Washington State University, Pullman, WA 99164 (USA)Takaaki Akaike - Department of Environmental Health Sciences and Molecular Toxicology, Tohoku University Graduate School of Medicine, Sendai, 980-8575 (Japan)Ming Xian - Department of Chemistry, Washington State University, Pullman, WA 99164 (USA). mxian@wsu.edu
- Publication Details
- Angewandte Chemie (International ed.), Vol.54(47), pp.13961-14171
- Academic Unit
- Chemistry, Department of
- Publisher
- Germany
- Grant note
- R01 HL116571 / NHLBI NIH HHS R01HL116571 / NHLBI NIH HHS
- Identifiers
- 99900547625401842
- Language
- English
- Resource Type
- Journal article