Journal article
The FLA3 KAP Subunit Is Required for Localization of Kinesin-2 to the Site of Flagellar Assembly and Processive Anterograde Intraflagellar Transport
Molecular biology of the cell, Vol.16(3), pp.1341-1354
03/2005
Handle:
https://hdl.handle.net/2376/108718
PMID: 15616187
Abstract
Intraflagellar transport (IFT) is a bidirectional process required for assembly and maintenance of cilia and flagella. Kinesin-2 is the anterograde IFT motor, and Dhc1b/Dhc2 drives retrograde IFT. To understand how either motor interacts with the IFT particle or how their activities might be coordinated, we characterized a ts mutation in the Chlamydomonas gene encoding KAP, the nonmotor subunit of Kinesin-2. The fla3-1 mutation is an amino acid substitution in a conserved C-terminal domain. fla3-1 strains assemble flagella at 21°C, but cannot maintain them at 33°C. Although the Kinesin-2 complex is present at both 21 and 33°C, the fla3-1 Kinesin-2 complex is not efficiently targeted to or retained in the basal body region or flagella. Video-enhanced DIC microscopy of fla3-1 cells shows that the frequency of anterograde IFT particles is significantly reduced. Anterograde particles move at near wild-type velocities, but appear larger and pause more frequently in fla3-1. Transformation with an epitope-tagged KAP gene rescues all of the fla3-1 defects and results in preferential incorporation of tagged KAP complexes into flagella. KAP is therefore required for the localization of Kinesin-2 at the site of flagellar assembly and the efficient transport of anterograde IFT particles within flagella.
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Details
- Title
- The FLA3 KAP Subunit Is Required for Localization of Kinesin-2 to the Site of Flagellar Assembly and Processive Anterograde Intraflagellar Transport
- Creators
- Joshua Mueller - Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455Catherine A Perrone - Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455Raqual Bower - Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455Douglas G Cole - Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, ID 83844-3052Mary E Porter - Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455
- Publication Details
- Molecular biology of the cell, Vol.16(3), pp.1341-1354
- Academic Unit
- Center for Reproductive Biology
- Identifiers
- 99900547248701842
- Language
- English
- Resource Type
- Journal article