Journal article
The Infrared Spectra of Bacillus Bacteria Part I: Vegetative Bacillus versus Sporulated Cells and the Contributions of Phospholipids to Vegetative Infrared Spectra
Applied spectroscopy, Vol.63(8), pp.899-907
08/2009
Handle:
https://hdl.handle.net/2376/110290
PMID: 19678986
Abstract
This paper highlights the distinctions between the infrared (IR) absorption spectra of vegetative versus sporulated Bacillus bacteria. It is observed that there are unique signatures clearly associated with either the sporulated or vegetative state and that vegetative cells (or cell debris) can contribute to the spore spectra. A distinct feature at ∼1739 cm−1 appears to be unique to vegetative cell spectra and can also be used as an indicator of vegetative cells or cell debris in the spore spectra. The data indicate that the band arises from a lipid-soluble species such as an ester or phospholipid carbonyl bond and are consistent with it being either phosphatidyl glycerol (PG) or phosphatidylethanolamine (PE), two major classes of phospholipids found in vegetative cells of Bacillus species. A companion work discusses bands associated with the sporulated state.
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Details
- Title
- The Infrared Spectra of Bacillus Bacteria Part I: Vegetative Bacillus versus Sporulated Cells and the Contributions of Phospholipids to Vegetative Infrared Spectra
- Creators
- Timothy J Johnson - Pacific Northwest National Laboratory, P.O. Box 999 Richland, Washington 99354 (T.J.J., Y.-F.S., N.B.V., H.W.K.-M., K.L.W., B.D.C., D.S.W.); and A. R. Smith Department of Chemistry, Appalachian State University, Boone, North Carolina 28608 (S.D.W.)Yin-Fong Su - Pacific Northwest National Laboratory, P.O. Box 999 Richland, Washington 99354 (T.J.J., Y.-F.S., N.B.V., H.W.K.-M., K.L.W., B.D.C., D.S.W.); and A. R. Smith Department of Chemistry, Appalachian State University, Boone, North Carolina 28608 (S.D.W.)Nancy B Valentine - Pacific Northwest National Laboratory, P.O. Box 999 Richland, Washington 99354 (T.J.J., Y.-F.S., N.B.V., H.W.K.-M., K.L.W., B.D.C., D.S.W.); and A. R. Smith Department of Chemistry, Appalachian State University, Boone, North Carolina 28608 (S.D.W.)Helen W Kreuzer-Martin - Pacific Northwest National Laboratory, P.O. Box 999 Richland, Washington 99354 (T.J.J., Y.-F.S., N.B.V., H.W.K.-M., K.L.W., B.D.C., D.S.W.); and A. R. Smith Department of Chemistry, Appalachian State University, Boone, North Carolina 28608 (S.D.W.)Karen L Wahl - Pacific Northwest National Laboratory, P.O. Box 999 Richland, Washington 99354 (T.J.J., Y.-F.S., N.B.V., H.W.K.-M., K.L.W., B.D.C., D.S.W.); and A. R. Smith Department of Chemistry, Appalachian State University, Boone, North Carolina 28608 (S.D.W.)Stephen D Williams - Pacific Northwest National Laboratory, P.O. Box 999 Richland, Washington 99354 (T.J.J., Y.-F.S., N.B.V., H.W.K.-M., K.L.W., B.D.C., D.S.W.); and A. R. Smith Department of Chemistry, Appalachian State University, Boone, North Carolina 28608 (S.D.W.)Brian H Clowers - Pacific Northwest National Laboratory, P.O. Box 999 Richland, Washington 99354 (T.J.J., Y.-F.S., N.B.V., H.W.K.-M., K.L.W., B.D.C., D.S.W.); and A. R. Smith Department of Chemistry, Appalachian State University, Boone, North Carolina 28608 (S.D.W.)David S Wunschel - Pacific Northwest National Laboratory, P.O. Box 999 Richland, Washington 99354 (T.J.J., Y.-F.S., N.B.V., H.W.K.-M., K.L.W., B.D.C., D.S.W.); and A. R. Smith Department of Chemistry, Appalachian State University, Boone, North Carolina 28608 (S.D.W.)
- Publication Details
- Applied spectroscopy, Vol.63(8), pp.899-907
- Academic Unit
- Chemistry, Department of
- Publisher
- SAGE Publications; London, England
- Identifiers
- 99900547350801842
- Language
- English
- Resource Type
- Journal article