Journal article
The Intestinal Microbiota Influences Campylobacter jejuni Colonization and Extraintestinal Dissemination in Mice
Applied and environmental microbiology, Vol.81(14), pp.4642-4650
07/2015
Handle:
https://hdl.handle.net/2376/108883
PMCID: PMC4551207
PMID: 25934624
Abstract
Campylobacter jejuni is a leading cause of human foodborne gastroenteritis worldwide. The interactions between this pathogen and the intestinal microbiome within a host are of interest as endogenous intestinal microbiota mediates a form of resistance to the pathogen. This resistance, termed colonization resistance, is the ability of commensal microbiota to prevent colonization by exogenous pathogens or opportunistic commensals. Although mice normally demonstrate colonization resistance to C. jejuni, we found that mice treated with ampicillin are colonized by C. jejuni, with recovery of Campylobacter from the colon, mesenteric lymph nodes, and spleen. Furthermore, there was a significant reduction in recovery of C. jejuni from ampicillin-treated mice inoculated with a C. jejuni virulence mutant (ΔflgL strain) compared to recovery of mice inoculated with the C. jejuni wild-type strain or the C. jejuni complemented isolate (ΔflgL/flgL). Comparative analysis of the microbiota from nontreated and ampicillin-treated CBA/J mice led to the identification of a lactic acid-fermenting isolate of Enterococcus faecalis that prevented C. jejuni growth in vitro and limited C. jejuni colonization of mice. Next-generation sequencing of DNA from fecal pellets that were collected from ampicillin-treated CBA/J mice revealed a significant decrease in diversity of operational taxonomic units (OTUs) compared to that in control (nontreated) mice. Taken together, we have demonstrated that treatment of mice with ampicillin alters the intestinal microbiota and permits C. jejuni colonization. These findings provide valuable insights for researchers using mice to investigate C. jejuni colonization factors, virulence determinants, or the mechanistic basis of probiotics.
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- Title
- The Intestinal Microbiota Influences Campylobacter jejuni Colonization and Extraintestinal Dissemination in Mice
- Creators
- Jason L O'Loughlin - School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, Pullman, Washington, USADerrick R Samuelson - School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, Pullman, Washington, USAAndrea G Braundmeier-Fleming - Department of Medical Microbiology, Immunology and Cell Biology, Southern Illinois University School of Medicine, Springfield, Illinois, USA Department of Animal Sciences, Division of Nutritional Sciences, University of Illinois, Urbana, Illinois, USABryan A White - Department of Animal Sciences, Division of Nutritional Sciences, University of Illinois, Urbana, Illinois, USAGary J Haldorson - Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, Washington, USAJennifer B Stone - School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, Pullman, Washington, USAJeremy J Lessmann - Department of Chemistry, College of Arts and Sciences, Washington State University, Pullman, Washington, USATyson P Eucker - School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, Pullman, Washington, USAMichael E Konkel - School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, Pullman, Washington, USA konkel@vetmed.wsu.edu
- Publication Details
- Applied and environmental microbiology, Vol.81(14), pp.4642-4650
- Academic Unit
- Chemistry, Department of; Veterinary Microbiology and Pathology, Department of; Molecular Biosciences, School of
- Publisher
- United States
- Grant note
- T32 AI007025 / NIAID NIH HHS 5 T32 AI 7025-33 / NIAID NIH HHS T32GM083864 / NIGMS NIH HHS T32GM008336 / NIGMS NIH HHS T32 GM083864 / NIGMS NIH HHS T32 GM008336 / NIGMS NIH HHS R56AI088518-01A1 / NIAID NIH HHS R56 AI088518 / NIAID NIH HHS
- Identifiers
- 99900546856101842
- Language
- English
- Resource Type
- Journal article