Journal article
The Mammalian Spermatogenesis Single-Cell Transcriptome, from Spermatogonial Stem Cells to Spermatids
Cell reports (Cambridge), Vol.25(6), pp.1650-1667.e8
11/06/2018
Handle:
https://hdl.handle.net/2376/113417
PMCID: PMC6384825
PMID: 30404016
Abstract
Spermatogenesis is a complex and dynamic cellular differentiation process critical to male reproduction and sustained by spermatogonial stem cells (SSCs). Although patterns of gene expression have been described for aggregates of certain spermatogenic cell types, the full continuum of gene expression patterns underlying ongoing spermatogenesis in steady state was previously unclear. Here, we catalog single-cell transcriptomes for >62,000 individual spermatogenic cells from immature (postnatal day 6) and adult male mice and adult men. This allowed us to resolve SSC and progenitor spermatogonia, elucidate the full range of gene expression changes during male meiosis and spermiogenesis, and derive unique gene expression signatures for multiple mouse and human spermatogenic cell types and/or subtypes. These transcriptome datasets provide an information-rich resource for studies of SSCs, male meiosis, testicular cancer, male infertility, or contraceptive development, as well as a gene expression roadmap to be emulated in efforts to achieve spermatogenesis in vitro.
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•Single-cell transcriptomes of >62,000 spermatogenic cells from mice and humans•Human SSC fate regulation pathways match those of functionally defined mouse SSCs•The hepatic stellate cell activation pathway is associated with SSC fate•Unique 3-gene identifiers distinguish 11 spermatogenic cell types in mice and humans
Hermann et al. present single-cell transcriptomes from >62,000 individual spermatogenic cells from immature and adult male mice and adult men. Their analysis facilitates resolution of SSCs and progenitor spermatogonia, elucidates the full range of gene expression changes during male meiosis and spermiogenesis, and derives unique gene expression signatures for eleven mouse and human spermatogenic cell types.
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Details
- Title
- The Mammalian Spermatogenesis Single-Cell Transcriptome, from Spermatogonial Stem Cells to Spermatids
- Creators
- Brian P Hermann - Department of Biology, University of Texas at San Antonio, San Antonio, TX 78249, USAKeren Cheng - Department of Biology, University of Texas at San Antonio, San Antonio, TX 78249, USAAnukriti Singh - Department of Biology, University of Texas at San Antonio, San Antonio, TX 78249, USALorena Roa-De La Cruz - Department of Biology, University of Texas at San Antonio, San Antonio, TX 78249, USAKazadi N Mutoji - Department of Biology, University of Texas at San Antonio, San Antonio, TX 78249, USAI-Chung Chen - Department of Biology, University of Texas at San Antonio, San Antonio, TX 78249, USAHeidi Gildersleeve - Genomics Core, University of Texas at San Antonio, San Antonio, TX 78249, USAJake D Lehle - Department of Biology, University of Texas at San Antonio, San Antonio, TX 78249, USAMax Mayo - Department of Biology, University of Texas at San Antonio, San Antonio, TX 78249, USABirgit Westernströer - Department of Biology, University of Texas at San Antonio, San Antonio, TX 78249, USANathan C Law - Center for Reproductive Biology, School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, Pullman, WA 99163, USAMelissa J Oatley - Center for Reproductive Biology, School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, Pullman, WA 99163, USAEllen K Velte - Department of Anatomy & Cell Biology, Brody School of Medicine, East Carolina University, Greenville, NC 27858, USABryan A Niedenberger - Department of Anatomy & Cell Biology, Brody School of Medicine, East Carolina University, Greenville, NC 27858, USADanielle Fritze - The UT Transplant Center, UT Health San Antonio, San Antonio, TX 78229, USASherman Silber - The Infertility Center of St. Louis, Chesterfield, MO 63017, USAChristopher B Geyer - Department of Anatomy & Cell Biology, Brody School of Medicine, East Carolina University, Greenville, NC 27858, USAJon M Oatley - Center for Reproductive Biology, School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, Pullman, WA 99163, USAJohn R McCarrey - Department of Biology, University of Texas at San Antonio, San Antonio, TX 78249, USA
- Publication Details
- Cell reports (Cambridge), Vol.25(6), pp.1650-1667.e8
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- Elsevier Inc
- Identifiers
- 99900547711001842
- Language
- English
- Resource Type
- Journal article