The Mre11 Complex Influences DNA Repair, Synapsis, and Crossing Over in Murine Meiosis

Sheila M Cherry; Carrie A Adelman; Jan W Theunissen; Terry J Hassold; Patricia A Hunt; John H.J Petrini

doi:10.1016/j.cub.2006.12.048

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The Mre11 Complex Influences DNA Repair, Synapsis, and Crossing Over in Murine Meiosis

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The Mre11 Complex Influences DNA Repair, Synapsis, and Crossing Over in Murine Meiosis

Sheila M Cherry, Carrie A Adelman, Jan W Theunissen, Terry J Hassold, Patricia A Hunt and John H.J Petrini

Current biology, Vol.17(4), pp.373-378

02/20/2007

DOI: https://doi.org/10.1016/j.cub.2006.12.048

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https://hdl.handle.net/2376/110763

PMCID: PMC1839861

PMID: 17291760

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Abstract

DNA

The Mre11 complex (consisting of MRE11, RAD50, and NBS1/Xrs2) is required for double-strand break (DSB) formation, processing, and checkpoint signaling during meiotic cell division in S. cerevisiae[1–8]. Whereas studies of Mre11 complex mutants in S. pombe and A. thaliana indicate that the complex has other essential meiotic roles [9–11], relatively little is known regarding the functions of the complex downstream of meiotic break formation and processing or its role in meiosis in higher eukaryotes. We analyzed meiotic events in mice harboring hypomorphic Mre11 and Nbs1 mutations which, unlike null mutants, support viability [12–16]. Our studies revealed defects in the temporal progression of meiotic prophase, incomplete and aberrant synapsis of homologous chromosomes, persistence of strand exchange proteins, and alterations in both the frequency and placement of MLH1 foci, a marker of crossovers. A unique sex-dependent effect on MLH1 foci and chiasmata numbers was observed: males exhibited an increase and females a decrease in recombination levels. Thus, our findings implicate the Mre11 complex in meiotic DNA repair and synapsis in mammals and indicate that the complex may contribute to the establishment of normal sex-specific differences in meiosis.

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Title: The Mre11 Complex Influences DNA Repair, Synapsis, and Crossing Over in Murine Meiosis
Creators: Sheila M Cherry - Department of Genetics, Case Western Reserve University, Cleveland, Ohio 44106
Carrie A Adelman - Molecular Biology and Genetics, Sloan-Kettering Institute and Weill-Cornell Graduate School of Medical Science, New York, New York 10021
Jan W Theunissen - Molecular Biology and Genetics, Sloan-Kettering Institute and Weill-Cornell Graduate School of Medical Science, New York, New York 10021
Terry J Hassold - School of Molecular Biosciences, Washington State University, Pullman, Washington 99164
Patricia A Hunt - School of Molecular Biosciences, Washington State University, Pullman, Washington 99164
John H.J Petrini - Molecular Biology and Genetics, Sloan-Kettering Institute and Weill-Cornell Graduate School of Medical Science, New York, New York 10021
Publication Details: Current biology, Vol.17(4), pp.373-378
Academic Unit: Molecular Biosciences, School of
Publisher: Elsevier Inc
Identifiers: 99900547036101842
Language: English
Resource Type: Journal article