Journal article
The Plant Microtubule-Associated Protein AtMAP65-3/PLE Is Essential for Cytokinetic Phragmoplast Function
Current biology, Vol.14(5), pp.412-417
03/09/2004
Handle:
https://hdl.handle.net/2376/109493
PMCID: PMC2867789
PMID: 15028217
Abstract
Directional cell expansion in interphase and nuclear and cell division in M-phase are mediated by four microtubule arrays, three of which are unique to plants: the interphase array, the preprophase band, and the phragmoplast. The plant microtubule-associated protein MAP65 has been identified as a key structural component in these arrays [1]. The Arabidopsis genome has nine MAP65 genes, and here we show that one, AtMAP65-3/PLE, locates only to the mitotic arrays and is essential for cytokinesis. The Arabidopsis pleiade (ple) alleles are single recessive mutations, and we show that these mutations are in the AtMAP65-3 gene. Moreover, these mutations cause C-terminal truncations that abolish microtubule binding. In the ple mutants the anaphase spindle is normal, and the cytokinetic phragmoplast can form but is distorted; not only is it wider, but the midline, the region where oppositely oriented microtubules overlap, is unusually expanded. Here we present data that demonstrate an essential role for AtMAP65-3/PLE in cytokinesis in plant cells.
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Details
- Title
- The Plant Microtubule-Associated Protein AtMAP65-3/PLE Is Essential for Cytokinetic Phragmoplast Function
- Creators
- Sabine Müller - The Institute of Applied Genetics, BOKU-University of Natural Resources and Applied Life Sciences, Muthgasse 18, A-1190 Vienna, AustriaAndrei Smertenko - The Integrative Cell Biology Laboratory, School of Biological and Biomedical Sciences, University of Durham, South Road, Durham DH1 3LE, United KingdomVera Wagner - The Institute of Applied Genetics, BOKU-University of Natural Resources and Applied Life Sciences, Muthgasse 18, A-1190 Vienna, AustriaMaria Heinrich - The Institute of Applied Genetics, BOKU-University of Natural Resources and Applied Life Sciences, Muthgasse 18, A-1190 Vienna, AustriaPatrick J Hussey - The Integrative Cell Biology Laboratory, School of Biological and Biomedical Sciences, University of Durham, South Road, Durham DH1 3LE, United KingdomMarie-Theres Hauser - The Institute of Applied Genetics, BOKU-University of Natural Resources and Applied Life Sciences, Muthgasse 18, A-1190 Vienna, Austria
- Publication Details
- Current biology, Vol.14(5), pp.412-417
- Academic Unit
- Biological Chemistry, Institute of
- Publisher
- Elsevier Inc
- Identifiers
- 99900547749801842
- Language
- English
- Resource Type
- Journal article