The RUB/Nedd8 conjugation pathway is required for early development in Arabidopsis

Sunethra Dharmasiri; Nihal Dharmasiri; Hanjo Hellmann; Mark Estelle

doi:10.1093/emboj/cdg190

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The RUB/Nedd8 conjugation pathway is required for early development in Arabidopsis

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The RUB/Nedd8 conjugation pathway is required for early development in Arabidopsis

Sunethra Dharmasiri, Nihal Dharmasiri, Hanjo Hellmann and Mark Estelle

The EMBO journal, Vol.22(8), pp.1762-1770

04/15/2003

DOI: https://doi.org/10.1093/emboj/cdg190

Handle:

https://hdl.handle.net/2376/110299

PMCID: PMC154480

PMID: 12682009

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Abstract

CUL1

RCE1

auxin

RUB conjugation

RUB modification

The r elated-to- ub iquitin (RUB) protein is post-translationally conjugated to the cullin subunit of the SCF (SKP1, Cullin, F-box) class of ubiquitin protein ligases. Although the precise biochemical function of RUB modification is unclear, studies indicate that the modification is important for SCF function. In Arabidopsis , RUB modification of CUL1 is required for normal function of SCF TIR1 , an E3 required for response to the plant hormone auxin. In this report we show that an Arabidopsis protein called RCE1 functions as a RUB-conjugating enzyme in vivo . A mutation in the RCE1 gene results in a phenotype like that of the axr1 mutant. Most strikingly, plants deficient in both RCE1 and AXR1 have an embryonic phenotype similar to mp and bdl mutants, previously shown to be deficient in auxin signaling. Based on these results, we suggest that the RUB-conjugation pathway is required for auxin-dependent pattern formation in the developing embryo. In addition, we show that RCE1 interacts directly with the RING protein RBX1 and is present in a stable complex with SCF. We propose that RBX1 functions as an E3 for RUB modification of CUL1.

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Title: The RUB/Nedd8 conjugation pathway is required for early development in Arabidopsis
Creators: Sunethra Dharmasiri - Department of Biology and Indiana Molecular Biology Institute, Indiana University, Bloomington, IN 47405 and
Nihal Dharmasiri - Department of Biology and Indiana Molecular Biology Institute, Indiana University, Bloomington, IN 47405 and
Hanjo Hellmann - Department of Biology and Indiana Molecular Biology Institute, Indiana University, Bloomington, IN 47405 and
Mark Estelle - Department of Biology and Indiana Molecular Biology Institute, Indiana University, Bloomington, IN 47405 and
Publication Details: The EMBO journal, Vol.22(8), pp.1762-1770
Academic Unit: Biological Sciences, School of
Publisher: Oxford University Press; Oxford, UK
Identifiers: 99900547238401842
Language: English
Resource Type: Journal article