Journal article
The effect of bovine serum albumin on batch and continuous enzymatic cellulose hydrolysis mixed by stirring or shaking
Bioresource technology, Vol.102(10), pp.6295-6298
2011
Handle:
https://hdl.handle.net/2376/112043
PMID: 21376571
Abstract
Bovine serum albumin (BSA) was applied as a model non-catalytic protein to enzymatic hydrolysis of Avicel and dilute acid pretreated corn stover at different reaction conditions to improve the understanding of its ability to enhance cellulose hydrolysis. Addition of BSA improved the 72
h hydrolysis yields in shake flasks by up to 26% for both substrates by reducing de-activation of the exoglucanases and by facilitating reductions in particle size and crystallinity during a magnetically stirred pre-incubation step. The enzyme stabilizing effect of BSA addition was most striking for batch hydrolysis in a stirred tank reactor, with glucose yields increasing by 76% after 72
h for Avicel and by 40% after 145
h for corn stover. Application of BSA to continuous hydrolysis for a mean residence time of 24
h gave 33% and 40% higher glucose yields for corn stover and Avicel compared to the controls.
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Details
- Title
- The effect of bovine serum albumin on batch and continuous enzymatic cellulose hydrolysis mixed by stirring or shaking
- Creators
- Simone Brethauer - Center for Environmental Research and Technology and Chemical and Environmental Engineering Department, University of California, 1084 Columbia Ave., Riverside, CA 92507, United StatesMichael H Studer - Center for Environmental Research and Technology and Chemical and Environmental Engineering Department, University of California, 1084 Columbia Ave., Riverside, CA 92507, United StatesBin Yang - Center for Environmental Research and Technology and Chemical and Environmental Engineering Department, University of California, 1084 Columbia Ave., Riverside, CA 92507, United StatesCharles E Wyman - Center for Environmental Research and Technology and Chemical and Environmental Engineering Department, University of California, 1084 Columbia Ave., Riverside, CA 92507, United States
- Publication Details
- Bioresource technology, Vol.102(10), pp.6295-6298
- Academic Unit
- Biological Systems Engineering, Department of
- Publisher
- Elsevier Ltd
- Identifiers
- 99900547316001842
- Language
- English
- Resource Type
- Journal article