The effect of the aquatic contaminants bisphenol-A and PCB-95 on the zebrafish lateral line

Lauren Hayashi; Meghal Sheth; Alexander Young; Matthew Kruger; Gary A Wayman; Allison B Coffin

doi:10.1016/j.neuro.2014.12.010

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The effect of the aquatic contaminants bisphenol-A and PCB-95 on the zebrafish lateral line

Journal article

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The effect of the aquatic contaminants bisphenol-A and PCB-95 on the zebrafish lateral line

Lauren Hayashi, Meghal Sheth, Alexander Young, Matthew Kruger, Gary A Wayman and Allison B Coffin

Neurotoxicology (Park Forest South), Vol.46, pp.125-136

01/2015

DOI: https://doi.org/10.1016/j.neuro.2014.12.010

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https://hdl.handle.net/2376/107128

PMCID: PMC5973882

PMID: 25556122

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https://doi.org/10.1016/j.neuro.2014.12.010View

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Abstract

Hair cell

Lateral line

Hearing

Bisphenol-A

Polychlorinated biphenyl

•Bisphenol-A (BPA) is toxic to sensory hair cells in the zebrafish lateral line.•BPA likely damages hair cells by inducing oxidative stress.•BPA attenuates hair cell regeneration in this innately regenerative system.•PCB-95 was not toxic to developing or mature lateral line hair cells. Environmental toxicants such as bisphenol-A (BPA) and polychlorinated biphenyls (PCBs) are prevalent in our water supply, soil, and many food products and can profoundly affect the central nervous system. Both BPA and PCBs can disrupt endocrine signaling, which is important for auditory development and function, but the effect of these toxicants on the auditory periphery is not understood. In this study we investigated the effect of PCB-95 and BPA on lateral line development, function, and regeneration in larval zebrafish. The lateral line is a system of mechanosensory hair cells on the exterior of the fish that are homologous to the hair cells located in the mammalian inner ear. We found that PCB-95 had no effect on lateral line development or hair cell survival. BPA also did not affect lateral line development, but instead had a significant effect on both hair cell survival and regeneration. BPA-induced hair cell loss is both dose- and time-dependent, with concentrations of 1μM or higher killing lateral line hair cells during a 24h exposure period. Pharmacologic manipulation experiments suggest that BPA kills hair cells via activation of oxidative stress pathways, similar to prior reports of BPA toxicity in other tissues. We also observed that hair cells killed with neomycin, a known ototoxin, failed to regenerate normally when BPA was present, suggesting that BPA in aquatic environments could impede innate regenerative responses in fishes. Collectively, these data demonstrate that BPA can have detrimental effects on sensory systems, both in aquatic life and perhaps in terrestrial organisms, including humans.

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Title: The effect of the aquatic contaminants bisphenol-A and PCB-95 on the zebrafish lateral line
Creators: Lauren Hayashi - College of Arts and Sciences, Washington State University, Vancouver, WA, USA
Meghal Sheth - College of Arts and Sciences, Washington State University, Vancouver, WA, USA
Alexander Young - College of Arts and Sciences, Washington State University, Vancouver, WA, USA
Matthew Kruger - College of Arts and Sciences, Washington State University, Vancouver, WA, USA
Gary A Wayman - Department of Integrative Physiology and Neuroscience, Washington State University, Pullman, WA, USA
Allison B Coffin - College of Arts and Sciences, Washington State University, Vancouver, WA, USA
Publication Details: Neurotoxicology (Park Forest South), Vol.46, pp.125-136
Academic Unit: Integrative Physiology and Neuroscience, Department of
Publisher: Elsevier B.V
Identifiers: 99900546859701842
Language: English
Resource Type: Journal article