Journal article
The effects of immunolesions of nerve growth factor-receptive neurons by 192 IgG-saporin on sleep
Brain research, Vol.712(1), pp.53-59
1996
Handle:
https://hdl.handle.net/2376/114215
PMID: 8705307
Abstract
Low-affinity nerve growth factor (NGF) receptors are present on the cholinergic neurons of the basal forebrain. We studied the effects of 192 IgG-saporin, a specific immunotoxin for the NGF receptor-positive, cholinergic basal forebrain neurons, on sleep, the power spectrum of the electroencephalogram (EEG), and body temperature. After 3 d baseline recordings, 12 male rats were injected intracerebroventricularly with 4 μg 192 IgG-saporin. EEG, motor activity, and brain temperature were recorded for 23 h on the first, third, fifth, and seventh day after the treatment. 192 IgG-saporin did not affect the total daily amounts but altered the circadian distribution of sleep. On days 1 and 3 after the injection of the immunotoxin, the amount of non-rapid-eye-movement sleep (NREMS) and rapid-eye-movement sleep (REMS) increased during the dark period, whereas during the light both NREMS and REMS decreased. On day 5, these changes were less pronounced and sleep completely returned to the baseline by day 7. The EEG was suppressed in each frequency band and each vigilance state, and, in contrast to sleep, these changes in EEG persisted for 7 days. Brain temperature was decreased from day 3. These results suggest that NGF receptor-positive, cholinergic basal forebrain neurons are not necessary for the maintenance of total sleep time but contribute to the generation of normal EEG and the maintenance of brain temperature.
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Details
- Title
- The effects of immunolesions of nerve growth factor-receptive neurons by 192 IgG-saporin on sleep
- Creators
- Levente Kapás - Department of Physiology and Biophysics, The University of Tennessee, Memphis, TN 38163, USAFerenc Obál - Department of Physiology, Albert Szent-Györgyi Medical University, Szeged, HungaryAdam A Book - Department of Anatomy and Neurobiology, The University of Tennessee, Memphis, IN 38163, USAJohn B. Schweitzer - Department of Pathology, The University of Tennessee, Memphis, TN 38163, USARonald G Wiley - Neurology Service, Disabled Veterans Affairs Medical Center, Nashville, TN 37212, USAJames M Krueger - Department of Physiology and Biophysics, The University of Tennessee, Memphis, TN 38163, USA
- Publication Details
- Brain research, Vol.712(1), pp.53-59
- Academic Unit
- Biomedical Sciences, Department of; Integrative Physiology and Neuroscience, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900547940001842
- Language
- English
- Resource Type
- Journal article