Journal article
The epsomitic phototrophic microbial mat of Hot Lake, Washington: community structural responses to seasonal cycling
Frontiers in microbiology, Vol.4, pp.323-323
2013
Handle:
https://hdl.handle.net/2376/103941
PMID: 24312082
Abstract
Phototrophic microbial mats are compact ecosystems composed of highly interactive organisms in which energy and element cycling take place over millimeter-to-centimeter-scale distances. Although microbial mats are common in hypersaline environments, they have not been extensively characterized in systems dominated by divalent ions. Hot Lake is a meromictic, epsomitic lake that occupies a small, endorheic basin in north-central Washington. The lake harbors a benthic, phototrophic mat that assembles each spring, disassembles each fall, and is subject to greater than tenfold variation in salinity (primarily Mg
2+
and SO
2−
4
) and irradiation over the annual cycle. We examined spatiotemporal variation in the mat community at five time points throughout the annual cycle with respect to prevailing physicochemical parameters by amplicon sequencing of the V4 region of the 16S rRNA gene coupled to near-full-length 16S RNA clone sequences. The composition of these microbial communities was relatively stable over the seasonal cycle and included dominant populations of
Cyanobacteria
, primarily a group IV cyanobacterium (
Leptolyngbya
), and
Alphaproteobacteria
(specifically, members of
Rhodobacteraceae
and
Geminicoccus
). Members of
Gammaproteobacteria
(e.g.,
Thioalkalivibrio
and
Halochromatium
) and
Deltaproteobacteria
(e.g.,
Desulfofustis
) that are likely to be involved in sulfur cycling peaked in summer and declined significantly by mid-fall, mirroring larger trends in mat community richness and evenness. Phylogenetic turnover analysis of abundant phylotypes employing environmental metadata suggests that seasonal shifts in light variability exert a dominant influence on the composition of Hot Lake microbial mat communities. The seasonal development and organization of these structured microbial mats provide opportunities for analysis of the temporal and physical dynamics that feed back to community function.
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Details
- Title
- The epsomitic phototrophic microbial mat of Hot Lake, Washington: community structural responses to seasonal cycling
- Creators
- Stephen R Lindemann - Biological Sciences Division, Fundamental and Computational Sciences Directorate, Pacific Northwest National LaboratoryJames J Moran - Chemical, Biological, and Physical Sciences Division, National Security Directorate, Pacific Northwest National LaboratoryJames C Stegen - Biological Sciences Division, Fundamental and Computational Sciences Directorate, Pacific Northwest National LaboratoryRyan S Renslow - Scientific Resources Division, William R. Wiley Environmental Molecular Sciences Laboratory, Pacific Northwest National LaboratoryJanine R Hutchison - Chemical, Biological, and Physical Sciences Division, National Security Directorate, Pacific Northwest National LaboratoryJessica K Cole - Biological Sciences Division, Fundamental and Computational Sciences Directorate, Pacific Northwest National LaboratoryAlice C Dohnalkova - Scientific Resources Division, William R. Wiley Environmental Molecular Sciences Laboratory, Pacific Northwest National LaboratoryJulien Tremblay - Lawrence Berkelely National Laboratory, Joint Genome InstituteKanwar Singh - Lawrence Berkelely National Laboratory, Joint Genome InstituteStephanie A Malfatti - Lawrence Berkelely National Laboratory, Joint Genome InstituteFeng Chen - Lawrence Berkelely National Laboratory, Joint Genome InstituteSusannah G Tringe - Lawrence Berkelely National Laboratory, Joint Genome InstituteHaluk Beyenal - The Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State UniversityJames K Fredrickson - Biological Sciences Division, Fundamental and Computational Sciences Directorate, Pacific Northwest National Laboratory
- Publication Details
- Frontiers in microbiology, Vol.4, pp.323-323
- Academic Unit
- Chemical Engineering and Bioengineering, School of
- Publisher
- Frontiers Media S.A
- Identifiers
- 99900546882601842
- Language
- English
- Resource Type
- Journal article