Journal article
The equine CD1 gene family is the largest and most diverse yet identified
Immunogenetics (New York), Vol.66(1), pp.33-42
01/2014
Handle:
https://hdl.handle.net/2376/105397
PMID: 24196432
Abstract
The CD1 family is a group of non-polymorphic MHC class I-like molecules that present lipid-based antigens to T cells. Previous work in our laboratory demonstrated that cytotoxic T lymphocytes from immune adult horses recognize lipids from the cell wall of an important equine pathogen, Rhodococcus equi. These findings suggest an important role for the equine CD1 antigen presentation system in protective immune responses to microbial pathogens in the horse. In this study, we characterized and mapped the equine CD1 gene cluster. The equine genome was found to contain 13 complete CD1 genes; seven genes were classified as homologues of human CD1a, two CD1b, one CD1c, one CD1d, and two CD1e, making it the largest CD1 family to date. All but one of the eqCD1 molecules were expressed in all antigen-presenting cells investigated. The major amino acid differences between equine CD1 isoforms are located in the predicted antigen binding site, suggesting that a variety of lipid antigens can be presented. R. equi survives and replicates within professional phagocytes by arresting phagosome maturation between the early endosome and late phagosome. Based on the absence of a tyrosine sorting motif in all eqCD1a, CD1a molecules are predicted to co-localize with R. equi in the early endosome. Here, they could acquire lipid antigen and present it to T lymphocytes. The extraordinarily large number of CD1 molecules in the horse may reflect their crucial role in immunity to R. equi.
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Details
- Title
- The equine CD1 gene family is the largest and most diverse yet identified
- Creators
- Robson Dossa - Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine Washington State University P.O. Box 647040 Pullman WA 99164-7040 USADebra Alperin - Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine Washington State University P.O. Box 647040 Pullman WA 99164-7040 USAMelissa Hines - College of Veterinary Medicine University of Tennessee 2407 River Drive Knoxville TN 37996-4546 USAStephen Hines - Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine Washington State University P.O. Box 647040 Pullman WA 99164-7040 USA
- Publication Details
- Immunogenetics (New York), Vol.66(1), pp.33-42
- Academic Unit
- Veterinary Medicine, College of
- Publisher
- Springer Berlin Heidelberg; Berlin/Heidelberg
- Identifiers
- 99900546757101842
- Language
- English
- Resource Type
- Journal article