Journal article
The gene encoding ammonia monooxygenase subunit A exists in three nearly identical copies in Nitrosospira sp. NpAV
FEMS microbiology letters, Vol.139(2), pp.181-188
1996
Handle:
https://hdl.handle.net/2376/113306
PMID: 8674986
Abstract
The gene encoding ammonia monooxygenase subunit A (AmoA) was found in three copies in the genome of the chemolithotrophic soil bacterium,
Nitrosospira sp. NpAV. The open reading frame and flanking regions of the three copies were isolated from digested and size fractionated genomic DNA using oligodeoxyribonucleotide primers and polymerase chain reaction. The three gene copies of
amoA were sequenced and the sequences compared to each other. The open reading frames and the upstream and downstream flanking regions were nearly identical in the three copies. All three copies were expressed in recombinant
Escherichia coli strains from the indigenous promoter producing a product of approximately 30 kDa. All
amoA copies encode 274 amino acid polypeptides which have similarity to the ammonia monooxygenase acetylene-binding protein from
Nitrosomonas europaea.
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Details
- Title
- The gene encoding ammonia monooxygenase subunit A exists in three nearly identical copies in Nitrosospira sp. NpAV
- Creators
- Jeanette M Norton - Department of Plants, Soils and Biometerology, Utah State University, Logan, UT 84322-4820, USAJackie M Low - Department of Plants, Soils and Biometerology, Utah State University, Logan, UT 84322-4820, USAMartin G Klotz - Department of Biology, University of Colorado, Denver, CO 80217-3364, USA
- Publication Details
- FEMS microbiology letters, Vol.139(2), pp.181-188
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- Elsevier B.V
- Identifiers
- 99900548205101842
- Language
- English
- Resource Type
- Journal article