Journal article
The heterotrimeric motor protein kinesin‐II localizes to the midpiece and flagellum of sea urchin and sand dollar sperm
Cell motility and the cytoskeleton, Vol.38(1), pp.29-37
1997
Handle:
https://hdl.handle.net/2376/106368
PMID: 9295139
Abstract
We have utilized immunoblotting and light microscopic immunofluorescent staining methods to examine the expression and localization of sea urchin kinesin‐II, a heterotrimeric plus end‐directed microtubule motor protein (previously referred to as KRP(85/95)), in sea urchin and sand dollar sperm. We demonstrate the presence of the 85 K and 115 K subunits of kinesin‐II in sperm and localize these proteins to the sperm flagella and midpiece. The kinesin‐II localization pattern is punctate and discontinuous, and in the flagella it is quite distinct from the continuous labeling present in sperm labeled with anti‐flagellar dynein. The kinesin‐II staining is largely insensitive to prefixation detergent extraction, suggesting that it is not associated with membranous elements in the sperm. In the midpiece the kinesin‐II staining is similar to the pattern present in sperm labeled with an anti‐centrosomal antibody. To our knowledge, this is the first localization of kinesin‐like proteins in mature sperm and corroborates the recent identification and localization of kinesin‐like proteins in the flagella and basal body of the unicellular green alga Chlamydomonas. We hypothesize that kinesin‐II in the sperm may play functional roles in intraflagellar transport and/or the formation of flagella during spermatogenesis. Cell Motil. Cytoskeleton 38:29–37, 1997. © 1997 Wiley‐Liss, Inc.
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Details
- Title
- The heterotrimeric motor protein kinesin‐II localizes to the midpiece and flagellum of sea urchin and sand dollar sperm
- Creators
- John H HensonDouglas G ColeColleen D RoesenerStephanie CapuanoRobert J MendolaJonathan M Scholey
- Publication Details
- Cell motility and the cytoskeleton, Vol.38(1), pp.29-37
- Academic Unit
- Center for Reproductive Biology
- Publisher
- Wiley Subscription Services, Inc., A Wiley Company; Hoboken
- Number of pages
- 9
- Grant note
- NIH (GM47693; IF32AR08173; GM50718) NSF (USE‐9250861; MCB‐9257856) Dana Internship, Dickinson College
- Identifiers
- 99900546781501842
- Language
- English
- Resource Type
- Journal article