Journal article
The human MLL gene: nucleotide sequence, homology to the Drosophila trx zinc-finger domain, and alternative splicing
DNA and cell biology, Vol.14(6), pp.475-483
06/1995
Handle:
https://hdl.handle.net/2376/115543
PMID: 7598802
Abstract
We have previously reported the cloning of several cDNAs corresponding to the MLL gene. The predicted primary amino acid sequence of two of these clones, 14p-18B and 14-7, reveals nearly complete identity with parts of the sequences of HRX, ALL-1, and Htrx-1, including a Zinc-finger region with homology to the Drosophila trithorax gene. However, we found that there is a stretch of 39 amino acids that is absent from 14p-18B when compared to ALL-1 and HRX. Another sequence of three amino acids is present in ALL-1, but is absent from 14p-18B and HRX. Nucleotide sequence examination reveals that these differences arise from alternative splicing, suggesting that MLL, HRX, and ALL-1 each represents a different alternative splicing product from the same gene. At least two cDNA clones, 14-7 and 14p-18C, correspond to incompletely processed transcripts including intron sequences. Northern blots using a subclone of 14p-18B revealed mRNA species of 14-16 kb in size in various human tissues. RNase protection assays show that the splice variant containing exon 8 and lacking a 9-bp extension 3' of exon 12 is predominantly expressed in hematopoietic cell lines.
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Details
- Title
- The human MLL gene: nucleotide sequence, homology to the Drosophila trx zinc-finger domain, and alternative splicing
- Creators
- D Mbangkollo - Section of Hematology and Oncology, University of Chicago, IL 60637, USAR BurnettN McCabeM ThirmanH GillH YuJ D RowleyM O Diaz
- Publication Details
- DNA and cell biology, Vol.14(6), pp.475-483
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- United States
- Grant note
- CA42557 / NCI NIH HHS CA38725 / NCI NIH HHS
- Identifiers
- 99900547959101842
- Language
- English
- Resource Type
- Journal article