The influence of non-nociceptive factors on hot-plate latency in rats

Amanda Gunn; Erin N Bobeck; Ceri Weber; Michael M Morgan

doi:10.1016/j.jpain.2010.06.011

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The influence of non-nociceptive factors on hot-plate latency in rats

Journal article

Open access

Peer reviewed

The influence of non-nociceptive factors on hot-plate latency in rats

Amanda Gunn, Erin N Bobeck, Ceri Weber and Michael M Morgan

The journal of pain, Vol.12(2), pp.222-227

02/2011

DOI: https://doi.org/10.1016/j.jpain.2010.06.011

Handle:

https://hdl.handle.net/2376/104124

PMCID: PMC3312470

PMID: 20797920

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https://doi.org/10.1016/j.jpain.2010.06.011View

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Abstract

Motor Activity - physiology

Pain Threshold - physiology

Hot Temperature - adverse effects

Rats

Behavior, Animal - physiology

Male

Reaction Time - physiology

Rats, Sprague-Dawley

Pain - psychology

Pain Measurement - methods

Animals

Pain - drug therapy

Pain - physiopathology

Pain Threshold - drug effects

Female

Disease Models, Animal

The hot plate is a widely used test to assess nociception. The effect of non-nociceptive factors (weight, sex, activity, habituation, and repeated testing) on hot-plate latency was examined. Comparison of body weight and hot-plate latency revealed a small but significant inverse correlation (light rats had longer latencies). Habituating rats to the test room for 1 hour prior to testing did not decrease hot-plate latency except for female rats tested on days 2 to 4. Hot-plate latency decreased with repeated daily testing, but this was not caused by a decrease in locomotor activity or learning to respond. Activity on the hot plate was consistent across all 4 trials, and prior exposure to a room-temperature plate caused a similar decrease in latency as rats tested repeatedly on the hot plate. Despite this decrease in baseline hot-plate latency, there was no difference in morphine antinociceptive potency. The present study shows that weight, habituation to the test room, and repeated testing can alter baseline hot-plate latency, but these effects are small and have relatively little impact on morphine antinociception. This manuscript shows that non-nociceptive factors such as body weight, habituation, and repeated testing can alter hot-plate latency, but these factors do not alter morphine potency. In sum, the hot-plate test is an easy to use and reliable method to assess supraspinally organized nociceptive responses.

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Details

Title: The influence of non-nociceptive factors on hot-plate latency in rats
Creators: Amanda Gunn - Department of Psychology, Washington State University Vancouver, Vancouver, Washington, USA
Erin N Bobeck
Ceri Weber
Michael M Morgan
Publication Details: The journal of pain, Vol.12(2), pp.222-227
Academic Unit: Psychology, Department of
Publisher: United States
Grant note: R01 DA015498 / NIDA NIH HHS DA015498 / NIDA NIH HHS R01 DA015498-06A2 / NIDA NIH HHS
Identifiers: 99900546749301842
Language: English
Resource Type: Journal article