The involvement of the Mre11/Rad50/Nbs1 complex in the generation of G-overhangs at human telomeres

Weihang Chai; Agnel J Sfeir; Hirotoshi Hoshiyama; Jerry W Shay; Woodring E Wright

doi:10.1038/sj.embor.7400600

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The involvement of the Mre11/Rad50/Nbs1 complex in the generation of G-overhangs at human telomeres

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The involvement of the Mre11/Rad50/Nbs1 complex in the generation of G-overhangs at human telomeres

Weihang Chai, Agnel J Sfeir, Hirotoshi Hoshiyama, Jerry W Shay and Woodring E Wright

EMBO reports, Vol.7(2), pp.225-230

02/2006

DOI: https://doi.org/10.1038/sj.embor.7400600

Handle:

https://hdl.handle.net/2376/109760

PMCID: PMC1369251

PMID: 16374507

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https://doi.org/10.1038/sj.embor.7400600View

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Abstract

Rad50

DNA double-strand break repair

Mre11

telomere end processing

Nbs1

telomere

3′ G-overhang

Scientific Report

A central function of telomeres is to prevent chromosome ends from being recognized as DNA double-strand breaks (DSBs). Several proteins involved in processing DSBs associate with telomeres, but the roles of these factors at telomeres are largely unknown. To investigate whether the Mre11/Rad50/Nbs1 (MRN) complex is involved in the generation of proper 3′ G-overhangs at human telomere ends, we used RNA interference to decrease expression of MRN and analysed their effects. Reduction of MRN resulted in a transient shortening of G-overhang length in telomerase-positive cells. The terminal nucleotides of both C- and G-rich strands remain unaltered in Mre11-diminished cells, indicating that MRN is not responsible for specifying the final end-processing event. The reduction in overhang length was not seen in telomerase-negative cells, but was observed after the expression of exogenous telomerase, which suggested that the MRN complex might be involved in the recruitment or action of telomerase.

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Title: The involvement of the Mre11/Rad50/Nbs1 complex in the generation of G-overhangs at human telomeres
Creators: Weihang Chai - Department of Cell Biology, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, Texas 75390-9039, USA
Agnel J Sfeir - Department of Cell Biology, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, Texas 75390-9039, USA
Hirotoshi Hoshiyama - Department of Cell Biology, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, Texas 75390-9039, USA
Jerry W Shay - Department of Cell Biology, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, Texas 75390-9039, USA
Woodring E Wright - Department of Cell Biology, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, Texas 75390-9039, USA
Publication Details: EMBO reports, Vol.7(2), pp.225-230
Academic Unit: UNKNOWN
Identifiers: 99900547105201842
Language: English
Resource Type: Journal article