The kinase LYK5 is a major chitin receptor in Arabidopsis and forms a chitin-induced complex with related kinase CERK1

Yangrong Cao; Yan Liang; Kiwamu Tanaka; Cuong T Nguyen; Robert P Jedrzejczak; Andrzej Joachimiak; Gary Stacey

doi:10.7554/eLife.03766

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The kinase LYK5 is a major chitin receptor in Arabidopsis and forms a chitin-induced complex with related kinase CERK1

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The kinase LYK5 is a major chitin receptor in Arabidopsis and forms a chitin-induced complex with related kinase CERK1

Yangrong Cao, Yan Liang, Kiwamu Tanaka, Cuong T Nguyen, Robert P Jedrzejczak, Andrzej Joachimiak and Gary Stacey

eLife, Vol.3

10/23/2014

DOI: https://doi.org/10.7554/eLife.03766

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https://hdl.handle.net/2376/115197

PMCID: PMC4356144

PMID: 25340959

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Abstract

Protein Kinases - metabolism

Protein Structure, Tertiary

Plant Immunity

Phosphorylation

Arabidopsis - enzymology

Signal Transduction

Protein Multimerization

Arabidopsis - immunology

Models, Molecular

Protein Kinases - chemistry

Receptors, Cell Surface - metabolism

Serine - metabolism

Arabidopsis Proteins - metabolism

Tyrosine - metabolism

Arabidopsis Proteins - chemistry

Chitin - metabolism

Protein Binding

Mutation

Protein-Serine-Threonine Kinases - metabolism

Chitin is a fungal microbe-associated molecular pattern recognized in Arabidopsis by a lysin motif receptor kinase (LYK), AtCERK1. Previous research suggested that AtCERK1 is the major chitin receptor and mediates chitin-induced signaling through homodimerization and phosphorylation. However, the reported chitin binding affinity of AtCERK1 is quite low, suggesting another receptor with high chitin binding affinity might be present. Here, we propose that AtLYK5 is the primary chitin receptor in Arabidopsis. Mutations in AtLYK5 resulted in a significant reduction in chitin response. However, AtLYK5 shares overlapping function with AtLYK4 and, therefore, Atlyk4/Atlyk5-2 double mutants show a complete loss of chitin response. AtLYK5 interacts with AtCERK1 in a chitin-dependent manner. Chitin binding to AtLYK5 is indispensable for chitin-induced AtCERK1 phosphorylation. AtLYK5 binds chitin at a much higher affinity than AtCERK1. The data suggest that AtLYK5 is the primary receptor for chitin, forming a chitin inducible complex with AtCERK1 to induce plant immunity.

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Title: The kinase LYK5 is a major chitin receptor in Arabidopsis and forms a chitin-induced complex with related kinase CERK1
Creators: Yangrong Cao - Division of Plant Sciences, National Center for Soybean Biotechnology, University of Missouri, Columbia, United States
Yan Liang - Division of Plant Sciences, National Center for Soybean Biotechnology, University of Missouri, Columbia, United States
Kiwamu Tanaka - Division of Plant Sciences, National Center for Soybean Biotechnology, University of Missouri, Columbia, United States
Cuong T Nguyen - Division of Plant Sciences, National Center for Soybean Biotechnology, University of Missouri, Columbia, United States
Robert P Jedrzejczak - Midwest Center for Structural Genomics, Argonne National Laboratory, Argonne, United States
Andrzej Joachimiak - Midwest Center for Structural Genomics, Argonne National Laboratory, Argonne, United States
Gary Stacey - Division of Plant Sciences, National Center for Soybean Biotechnology, University of Missouri, Columbia, United States
Publication Details: eLife, Vol.3
Academic Unit: Plant Pathology, Department of
Publisher: England
Grant note: U54 GM094585 / NIGMS NIH HHS GM094585 / NIGMS NIH HHS
Identifiers: 99900547343001842
Language: English
Resource Type: Journal article