Journal article
The kinetic basis for age-associated changes in quercetin and genistein glucuronidation by rat liver microsomes
The Journal of nutritional biochemistry, Vol.21(6), pp.498-503
2010
Handle:
https://hdl.handle.net/2376/105368
PMCID: PMC2872046
PMID: 19446449
Abstract
The dietary bioavailability of the isoflavone genistein is decreased in older rats compared to young adults. Since flavonoids are metabolized extensively by the UDP-glucuronosyltransferases (UGTs), we hypothesized that UGT flavonoid conjugating activity changes with age. The effect of age on flavonoid glucuronidation was determined using hepatic microsomes from male F344 rats. Kinetic models of UGT activity toward the flavonol quercetin and the isoflavone genistein were established using pooled hepatic microsomal fractions of rats at different ages, and glucuronidation rates were determined using individual samples. Intrinsic clearance (
V
max/
K
m) values in 4-, 18- and 28-month-old rats were 0.100, 0.078 and 0.087 ml/min/mg for quercetin-7-
O-glucuronide; 0.138, 0.133 and 0.088 for quercetin-3′-
O-glucuronide; and 0.075, 0.077 and 0.057 for quercetin-4′-
O-glucuronide, respectively. While there were no differences in formation rates of total quercetin glucuronides in individual samples, the production of the primary metabolite, quercetin-7-
O-glucuronide, at 30 μM quercetin concentration was increased from 3.4 and 3.1 nmol/min/mg at 4 and 18 months to 3.8 nmol/min/mg at 28 months, while quercetin-3′-
O-glucuronide formation at 28 months declined by a similar degree (
P≤.05). At 30 and 300 μM quercetin concentration, the rate of quercetin-4′-
O-glucuronide formation peaked at 18 months at 0.9 nmol/min/mg. Intrinsic clearance values of genistein 7-
O-glucuronide increased with age, in contrast to quercetin glucuronidation. Thus, the capacity for flavonoid glucuronidation by rat liver microsomes is dependent on age, UGT isoenzymes and flavonoid structure.
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Details
- Title
- The kinetic basis for age-associated changes in quercetin and genistein glucuronidation by rat liver microsomes
- Creators
- Bradley W Bolling - Antioxidants Research Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111, USAMichael H Court - Department of Pharmacology, Tufts University, Boston, MA 02111, USAJeffrey B Blumberg - Antioxidants Research Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111, USAC.-Y. Oliver Chen - Antioxidants Research Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111, USA
- Publication Details
- The Journal of nutritional biochemistry, Vol.21(6), pp.498-503
- Academic Unit
- Veterinary Clinical Sciences, Department of
- Publisher
- Elsevier Inc
- Identifiers
- 99900546836301842
- Language
- English
- Resource Type
- Journal article