Journal article
The mechanism of neutral red-mediated microbial electrosynthesis in Escherichia coli: menaquinone reduction
Bioresource technology, Vol.192(C), pp.689-695
09/2015
Handle:
https://hdl.handle.net/2376/105342
PMCID: PMC4516386
PMID: 26094195
Abstract
•The mechanism of electro-synthesis with neutral red in E. coli was identified.•Neutral red reduces menaquinone in the inner membrane.•Resulting menaquinol drives anaerobic respiration with multiple electron acceptors.•Sans electron acceptor, menaquinol reduces the arcB redox sensor.•Reduced arcB causes transcriptional changes that alter metabolite profiles.
The aim of this work was to elucidate the mechanism of mediated microbial electrosynthesis via neutral red from an electrode to fermenting Escherichia coli cultures in a bioelectrochemical system. Chemical reduction of NAD+ by reduced neutral red did not occur as predicted. Instead, neutral red was shown to reduce the menaquinone pool in the inner bacterial membrane. The reduced menaquinone pool altered fermentative metabolite production via the arcB redox-sensing cascade in the absence of terminal electron acceptors. When the acceptors DMSO, fumarate, or nitrate were provided, as many as 19% of the electrons trapped in the reduced acceptors were derived from the electrode. These results demonstrate the mechanism of neutral red-mediated microbial electrosynthesis during fermentation as well as how neutral red enables microbial electrosynthesis of reduced terminal electron acceptors.
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Details
- Title
- The mechanism of neutral red-mediated microbial electrosynthesis in Escherichia coli: menaquinone reduction
- Creators
- Timothy D Harrington - The Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA, USAVi N Tran - The Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA, USAAbdelrhman Mohamed - The Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA, USARyan Renslow - Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, USASaeid Biria - The Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA, USALisa Orfe - Paul G. Allen School for Global Animal Health, Washington State University, Pullman, WA, USADouglas R Call - Paul G. Allen School for Global Animal Health, Washington State University, Pullman, WA, USAHaluk Beyenal - The Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA, USA
- Publication Details
- Bioresource technology, Vol.192(C), pp.689-695
- Academic Unit
- Chemical Engineering and Bioengineering, School of
- Publisher
- Elsevier Ltd
- Identifiers
- 99900546505601842
- Language
- English
- Resource Type
- Journal article