Journal article
The neuron-specific interleukin-1 receptor accessory protein is required for homeostatic sleep and sleep responses to influenza viral challenge in mice
Brain, behavior, and immunity, Vol.47, pp.35-43
07/2015
Handle:
https://hdl.handle.net/2376/106963
PMCID: PMC4418942
PMID: 25449578
Abstract
Interleukin-1β (IL1) is involved in sleep regulation and sleep responses induced by influenza virus. The IL1 receptor accessory protein (AcP) and an alternatively spliced isoform of AcP found primarily in neurons, AcPb, form part of the IL1 signaling complex. IL1-induced sleep responses depend on injection time. In rat cortex, both IL1 mRNA and AcPb mRNA peak at Zeitgeber Time (ZT) 0 then decline over the daylight hours. Sleep deprivation enhances cortical IL1 mRNA and AcPb mRNA levels, but not AcP mRNA. We used wild type (WT) and AcPb knockout (KO) mice and performed sleep deprivation between ZT10 and 20 or between ZT22 and 8 based on the time of day expression profiles of AcPb and IL1. We hypothesized that the magnitude of the responses to sleep loss would be strain- and time of day-dependent. In WT mice, NREMS and REMS rebounds occurred regardless of when they were deprived of sleep. In contrast, when AcPbKO mice were sleep deprived from ZT10 to 20 NREMS and REMS rebounds were absent. The AcPbKO mice expressed sleep rebound if sleep loss occurred from ZT22 to 8 although the NREMS responses were not as robust as those that occurred in WT mice. We also challenged mice with intranasal H1N1 influenza virus. WT mice exhibited the expected enhanced sleep responses. In contrast, the AcPbKO mice had less sleep after influenza challenge compared to their own baseline values and compared to WT mice. Body temperature and locomotor activity responses after viral challenge were lower and mortality was higher in AcPbKO than in WT mice. We conclude that neuron-specific AcPb plays a critical role in host defenses and sleep homeostasis.
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Details
- Title
- The neuron-specific interleukin-1 receptor accessory protein is required for homeostatic sleep and sleep responses to influenza viral challenge in mice
- Creators
- Christopher J Davis - College of Medical Sciences and the Sleep and Performance Research Center, Washington State University Spokane, Spokane, WA 99210, United States. Electronic address: cjdavis@wsu.eduDanielle Dunbrasky - College of Medical Sciences and the Sleep and Performance Research Center, Washington State University Spokane, Spokane, WA 99210, United StatesMarcella Oonk - College of Medical Sciences and the Sleep and Performance Research Center, Washington State University Spokane, Spokane, WA 99210, United StatesPing Taishi - College of Medical Sciences and the Sleep and Performance Research Center, Washington State University Spokane, Spokane, WA 99210, United StatesMark R Opp - Department of Anesthesiology & Pain Medicine, University of Washington, Seattle, WA 98104, United StatesJames M Krueger - College of Medical Sciences and the Sleep and Performance Research Center, Washington State University Spokane, Spokane, WA 99210, United States
- Publication Details
- Brain, behavior, and immunity, Vol.47, pp.35-43
- Academic Unit
- Biomedical Sciences, Department of; Integrative Physiology and Neuroscience, Department of
- Publisher
- Netherlands
- Grant note
- R01 NS025378 / NINDS NIH HHS NS025378 / NINDS NIH HHS R01 AG041287 / NIA NIH HHS HD036520 / NICHD NIH HHS R01 HD036520 / NICHD NIH HHS AG041287 / NIA NIH HHS
- Identifiers
- 99900546959201842
- Language
- English
- Resource Type
- Journal article