Journal article
The nuclear architectural protein HMGA1a triggers receptor‐mediated endocytosis
Journal of cellular biochemistry, Vol.108(4), pp.791-801
11/01/2009
Handle:
https://hdl.handle.net/2376/116680
PMID: 19739099
Abstract
High mobility group proteins A (HMGA), nuclear architectural factors, locate in the cell nuclei and mostly execute gene‐regulation function. However, our results reveal that a HMGA member (HMGA1a) has a unique plasma membrane receptor; this receptor specifically binds to HMGA‐decorated species, effectively mediates endocytosis, and internalizes extracellular HMGA‐functionalized cargoes. Indeed, dyes or nanoparticles labeled with HMGA1a protein readily enter Hela cells. Using a stratagem chemical cross‐linker, we covalently bonded the HMGA receptor to the HMGA1a‐GFP fusion protein, thus capturing the plasma membrane receptor. Subsequent Western blots and SDS–PAGE gel revealed that the HMGA receptor is a 26‐kDa protein. Confocal live‐cell microscopic imaging was used to monitor the whole endocytic process, in which the internalized HMGA1a‐decorated species are transported by motor proteins on microtubules and eventually arrive at the late endosomes/lysosomes. Cell viability assays also suggested that extracellular HMGA1a protein directly influences the survival ability of Hela cells in a dose‐dependent manner, implying versatility of HMGA1a protein and its potent role to suppress cancer cell survivability and to regulate growth. J. Cell. Biochem. 108: 791–801, 2009. © 2009 Wiley‐Liss, Inc.
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Details
- Title
- The nuclear architectural protein HMGA1a triggers receptor‐mediated endocytosis
- Creators
- Wuwei WuWei WanAlexander D.Q Li
- Publication Details
- Journal of cellular biochemistry, Vol.108(4), pp.791-801
- Academic Unit
- Department of Chemistry
- Publisher
- Wiley Subscription Services, Inc., A Wiley Company; Hoboken
- Number of pages
- 11
- Grant note
- National Institute of General Medicine Sciences (GM065306)
- Identifiers
- 99900547756001842
- Language
- English
- Resource Type
- Journal article