Journal article
The pig p160 co-activator family: Full length cDNA cloning, expression and effects on intramuscular fat content in Longissimus Dorsi muscle
Domestic animal endocrinology, Vol.35(2), pp.208-216
2008
Handle:
https://hdl.handle.net/2376/107376
PMID: 18638664
Abstract
Evidence has shown in mouse that the p160 co-activator family, consisting of nuclear receptor co-activators 1 (NCOA1), 2 (NCOA2) and 3 (NCOA3) plays a critical role in adipogenesis. In the present study, we cloned these three porcine genes, identified their transcript variants and analyzed their expression level in relation to intramuscular fat (IMF) content in
Longissimus Dorsi (LD) muscle. Both
in silico cloning and PCR amplification revealed a full length cDNA sequence of 6591
bp for NCOA1 (EU346671), 7628
bp for NCOA2 (EU346672) and 5005
bp for NCOA3 (EU346674) in pigs, respectively. Interestingly, three transcript variants were identified for the porcine
NCOA1 and two for the porcine
NCOA2 gene. In addition, the deduced amino acid sequences indicated that isoform 2 of
NCOA2 lacks the fourth LXXLL motif, the number of which has been shown to influence the selectivity and affinity for different nuclear receptors. Finally, 15 animals with high IMF content and fifteen animals with low IMF content (
p
<
0.05) selected from 60 individuals were used to investigate how the family members and their variants affect the phenotype in pigs using real-time PCR. Our results showed that both
NCOA1 transcript variant 2 (
r
=
−0.554,
p
<
0.01) and total
NCOA1 (
r
=
−0.516,
p
<
0.01) expression levels were negatively correlated with IMF contents, while
NCOA2 transcript variant 1 (
r
=
0.605,
p
<
0.01) and
NCOA3 (
r
=
0.435,
p
<
0.05) were positively associated with IMF content in LD muscle. Overall, the present study provides evidence for the first time that the p160 co-activator family might have a concordant effect on lipid metabolism in mammals.
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Details
- Title
- The pig p160 co-activator family: Full length cDNA cloning, expression and effects on intramuscular fat content in Longissimus Dorsi muscle
- Creators
- Xiuxing Wang - College of Animal Science & Technology, Nanjing Agricultural University, Weigang 1, Nanjing 210095, ChinaJie Chen - College of Animal Science & Technology, Nanjing Agricultural University, Weigang 1, Nanjing 210095, ChinaHonglin Liu - College of Animal Science & Technology, Nanjing Agricultural University, Weigang 1, Nanjing 210095, ChinaYinxue Xu - College of Animal Science & Technology, Nanjing Agricultural University, Weigang 1, Nanjing 210095, ChinaXiaona Wang - College of Animal Science & Technology, Nanjing Agricultural University, Weigang 1, Nanjing 210095, ChinaChunyang Xue - College of Animal Science & Technology, Nanjing Agricultural University, Weigang 1, Nanjing 210095, ChinaDebing Yu - College of Animal Science & Technology, Nanjing Agricultural University, Weigang 1, Nanjing 210095, ChinaZhihua Jiang - Department of Animal Sciences, Washington State University, Pullman 99164-6351, USA
- Publication Details
- Domestic animal endocrinology, Vol.35(2), pp.208-216
- Academic Unit
- Animal Sciences, Department of
- Publisher
- Elsevier Inc
- Identifiers
- 99900547372401842
- Language
- English
- Resource Type
- Journal article