The postimplantation embryo differentially regulates endometrial gene expression and decidualization

Aki Kashiwagi; Carla M DiGirolamo; Yoshiaki Kanda; Yuichi Niikura; Charles T Esmon; Thomas R Hansen; Toshi Shioda; James K Pru

doi:10.1210/en.2007-0268

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The postimplantation embryo differentially regulates endometrial gene expression and decidualization

Journal article

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The postimplantation embryo differentially regulates endometrial gene expression and decidualization

Aki Kashiwagi, Carla M DiGirolamo, Yoshiaki Kanda, Yuichi Niikura, Charles T Esmon, Thomas R Hansen, Toshi Shioda and James K Pru

Endocrinology (Philadelphia), Vol.148(9), pp.4173-4184

09/2007

DOI: https://doi.org/10.1210/en.2007-0268

Handle:

https://hdl.handle.net/2376/107528

PMID: 17510242

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Abstract

Immunohistochemistry

Endometrium - physiology

Oligonucleotide Array Sequence Analysis

Decidua - cytology

Humans

Gene Expression Regulation

Embryonic Development - physiology

DNA Primers

Mice, Inbred ICR

Pregnancy

Animals

Polymerase Chain Reaction

Decidua - physiology

Female

Mice

Endometrium - cytology

Transcriptomal changes in the uterine endometrium induced in response to the implanting embryo remain largely unknown. In this study, using Affymetrix mRNA expression microarray analysis, we identified genes differentially expressed in the murine endometrium in the presence or absence of the embryo. Compared with the pseudopregnant deciduoma induced by a mechanical stimulus in the absence of an embryo, approximately 1500 genes (753 up-regulated, 686 down-regulated; P < 0.05) were differentially expressed by at least 1.2-fold in the uterine decidua of pregnancy. Most of these genes fall into five major biological categories that include binding (45%), catalysis (24%), signal transduction (10%), transcriptional regulators (5%), and transporters (5%). This strong, embryo-induced transcriptomal impact represented approximately 10% of the total number of genes expressed in the decidualizing endometrium. Validation studies with mRNA and protein confirmed existence of the phylogenetically conserved, embryo-regulated genes involved in the following: 1) hemostasis and inflammation; 2) interferon signaling; 3) tissue growth and remodeling; and 4) natural killer cell function. Interestingly, whereas expression of many growth factors and their cognate receptors were not different between the decidual and deciduomal endometria, a number of proteases that degrade growth factors were selectively up-regulated in the decidual tissue. Increased expression of IGF and activin A neutralizing factors (i.e. HtrA1 and Fstl3) correlated with reduced stromal cell mitosis, tissue growth, and mitogenic signaling in the decidual endometrium. These results support the hypothesis that the implanting murine embryo takes a proactive role in modulating endometrial gene expression and development during early gestation.

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Title: The postimplantation embryo differentially regulates endometrial gene expression and decidualization
Creators: Aki Kashiwagi - Vincent Center for Reproductive Biology, Vincent Obstetrics and Gynecology Service, Massachusetts General Hospital, Harvard Medical School, Thier Research Building, Room 931, 55 Fruit Street, Boston, Massachusetts 02114, USA
Carla M DiGirolamo
Yoshiaki Kanda
Yuichi Niikura
Charles T Esmon
Thomas R Hansen
Toshi Shioda
James K Pru
Publication Details: Endocrinology (Philadelphia), Vol.148(9), pp.4173-4184
Academic Unit: Animal Sciences, Department of
Publisher: United States
Grant note: HD032475 / NICHD NIH HHS P50 HL54502 / NHLBI NIH HHS ES012070 / NIEHS NIH HHS
Identifiers: 99900546914001842
Language: English
Resource Type: Journal article