Journal article
The potential of desferrioxamine-gallium as an anti-Pseudomonas therapeutic agent
Proceedings of the National Academy of Sciences - PNAS, Vol.105(43), pp.16761-16766
10/28/2008
Handle:
https://hdl.handle.net/2376/109449
PMCID: PMC2575493
PMID: 18931304
Abstract
The opportunistic pathogen
Pseudomonas aeruginosa
causes infections that are difficult to treat by antibiotic therapy. This bacterium can cause biofilm infections where it shows tolerance to antibiotics. Here we report the novel use of a metallo-complex, desferrioxamine-gallium (DFO-Ga) that targets
P. aeruginosa
iron metabolism. This complex kills free-living bacteria and blocks biofilm formation. A combination of DFO-Ga and the anti-
Pseudomonas
antibiotic gentamicin caused massive killing of
P. aeruginosa
cells in mature biofilms. In a
P. aeruginosa
rabbit corneal infection, topical administration of DFO-Ga together with gentamicin decreased both infiltrate and final scar size by about 50% compared to topical application of gentamicin alone. The use of DFO-Ga as a Trojan horse delivery system that interferes with iron metabolism shows promise as a treatment for
P. aeruginosa
infections.
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Details
- Title
- The potential of desferrioxamine-gallium as an anti-Pseudomonas therapeutic agent
- Creators
- Ehud Banin - Department of Microbiology, School of Medicine, University of Washington, Seattle, WA 98195Alina Lozinski - Department of Ophthalmology, Hadassah-Hebrew University Medical Center, Jerusalem 91120, IsraelKeith M Brady - Department of Microbiology, Roy and Lucille Carver College of Medicine, University of Iowa, Iowa City, IA 52242Eduard Berenshtein - Department of Cellular Biochemistry and Human Genetics, The Hebrew University of Jerusalem, Jerusalem 91120, Israel; andPhillip W Butterfield - Department of Civil and Environmental Engineering, Washington State University, Spokane, WA 99210Maya Moshe - Institute for Nanotechnology and Advanced Materials, The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, IsraelMordechai Chevion - Department of Cellular Biochemistry and Human Genetics, The Hebrew University of Jerusalem, Jerusalem 91120, Israel; andEverett Peter Greenberg - Department of Microbiology, School of Medicine, University of Washington, Seattle, WA 98195Eyal Banin - Department of Ophthalmology, Hadassah-Hebrew University Medical Center, Jerusalem 91120, Israel
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.105(43), pp.16761-16766
- Publisher
- National Academy of Sciences
- Identifiers
- 99900547386501842
- Language
- English
- Resource Type
- Journal article