Journal article
The potential of enzyme recycling during the hydrolysis of a mixed softwood feedstock
Bioresource technology, Vol.100(24), pp.6407-6415
2009
Handle:
https://hdl.handle.net/2376/112425
PMID: 19632826
Abstract
Despite recent improvement in cellulase enzymes properties, the high cost associated with the hydrolysis step remains a major impediment to the commercialization of full-scale lignocellulose-to-ethanol bioconversion process. As part of a research effort to develop a commercial process for bioconversion of softwood residues, we have examined the potential for recycling enzymes during the hydrolysis of mixed softwood substrate pretreated by organosolv process. We have used response surface methodology to determine the optimal temperature, pH, ionic strength, and surfactant (Tween 80) concentration for maximizing the recovery of bound protein and enzyme activity from the residual substrates after hydrolysis. Data analysis showed that the temperature, pH and surfactant concentration were the major factors governing enzyme desorption from residual substrate. The optimized conditions were temperature 44.4
°C, pH 5.3 and 0.5% Tween 80. The optimal conditions significantly increased the hydrolysis yield by 25% after three rounds of hydrolysis. This bound enzyme desorption combining with free enzyme re-adsorption is a potential method to recover cellulase enzymes and reduce the cost of enzymatic hydrolysis.
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Details
- Title
- The potential of enzyme recycling during the hydrolysis of a mixed softwood feedstock
- Creators
- Maobing Tu - School of Forestry and Wildlife Sciences, Auburn University, Auburn, AL 36849-5418, USAXiao Zhang - FPInnovations, Pulp and Paper Research Institute of Canada (Paprican), 570 Boul. Saint Jean, Pointe-Claire, Quebec, Canada H9R 3J9Mike Paice - FPInnovations, Pulp and Paper Research Institute of Canada (Paprican), 570 Boul. Saint Jean, Pointe-Claire, Quebec, Canada H9R 3J9Paul MacFarlane - Department of Wood Science, University of British Columbia, Vancouver, BC, Canada V6T 1Z4Jack N Saddler - Department of Wood Science, University of British Columbia, Vancouver, BC, Canada V6T 1Z4
- Publication Details
- Bioresource technology, Vol.100(24), pp.6407-6415
- Academic Unit
- Chemical Engineering and Bioengineering, School of
- Publisher
- Elsevier Ltd
- Identifiers
- 99900547601101842
- Language
- English
- Resource Type
- Journal article