The proteins secreted by Trichomonas vaginalis and vaginal epithelial cell response to secreted and episomally expressed AP65

Ashwini S Kucknoor; Vasanthakrishna Mundodi; John F Alderete

doi:10.1111/j.1462-5822.2007.00979.x

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The proteins secreted by Trichomonas vaginalis and vaginal epithelial cell response to secreted and episomally expressed AP65

Journal article

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Peer reviewed

The proteins secreted by Trichomonas vaginalis and vaginal epithelial cell response to secreted and episomally expressed AP65

Ashwini S Kucknoor, Vasanthakrishna Mundodi and John F Alderete

Cellular microbiology, Vol.9(11), pp.2586-2597

11/2007

DOI: https://doi.org/10.1111/j.1462-5822.2007.00979.x

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https://hdl.handle.net/2376/116381

PMCID: PMC2574865

PMID: 17590165

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https://doi.org/10.1111/j.1462-5822.2007.00979.xView

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Abstract

Interleukin-8 - genetics

Trichomonas vaginalis - metabolism

Epithelial Cells - metabolism

Gene Expression - drug effects

Thioredoxin-Disulfide Reductase - genetics

Epithelial Cells - drug effects

Humans

Culture Media, Conditioned - pharmacology

Electrophoresis, Gel, Two-Dimensional

Protozoan Proteins - pharmacology

Cell Adhesion Molecules - immunology

Cysteine Endopeptidases - metabolism

Protozoan Proteins - metabolism

Thioredoxin-Disulfide Reductase - metabolism

Cyclooxygenase 2 - genetics

Cell Adhesion Molecules - pharmacology

Female

Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Interleukin-8 - metabolism

Epithelial Cells - cytology

Antibodies, Monoclonal - immunology

Vagina - cytology

Antibodies, Monoclonal - pharmacology

Electrophoresis, Polyacrylamide Gel

Cells, Cultured

Reverse Transcriptase Polymerase Chain Reaction

Cell Adhesion Molecules - metabolism

Fibronectins - metabolism

Animals

Cyclooxygenase 2 - metabolism

Fibronectins - genetics

HeLa Cells

Protozoan Proteins - immunology

Culture Media, Conditioned - metabolism

We showed recently that contact of human vaginal epithelial cells (VECs) by Trichomonas vaginalis and incubation with trichomonad proteins in conditioned medium induced expression of VEC genes. We performed 2-D SDS-PAGE followed by MALDI-TOF to identify the major secreted proteins. Based on protein abundance and separation of spots in 2-D gels, 32 major secreted proteins were examined, which gave 19 proteins with accession numbers. These proteins included known secreted cysteine proteinases. In addition, other secreted proteins were enzymes of carbohydrate metabolism, adhesin protein AP65, heat shock proteins, thioredoxin reductase and coronins. We confirmed that the secreted trichomonad proteins induced expression of VEC genes, including interleukin 8 (IL-8), COX-2 and fibronectin. Purified AP65 added to VECs had a pronounced effect only on IL-8 gene expression, which was inhibited in the presence of 12G4 monoclonal antibody to AP65. Moreover, AP65 expressed episomally within epithelial cells was found to enhance the expression of IL-8 and COX-2. This may be the first report of analysis of the secreted proteins of T. vaginalis and of the host epithelial cell response to these proteins and to the prominent adhesin AP65.

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Title: The proteins secreted by Trichomonas vaginalis and vaginal epithelial cell response to secreted and episomally expressed AP65
Creators: Ashwini S Kucknoor - Department of Microbiology and Immunology, University of Texas Health Science Center at San Antonio, TX, USA
Vasanthakrishna Mundodi
John F Alderete
Publication Details: Cellular microbiology, Vol.9(11), pp.2586-2597
Academic Unit: Molecular Biosciences, School of
Publisher: England
Grant note: AI43940 / NIAID NIH HHS R01 AI043940 / NIAID NIH HHS U19 AI045429 / NIAID NIH HHS R01 AI043940-04 / NIAID NIH HHS R21 AI043940 / NIAID NIH HHS U19 AI045429-080007 / NIAID NIH HHS AI45429 / NIAID NIH HHS
Identifiers: 99900547766901842
Language: English
Resource Type: Journal article