Journal article
The regulation of translation by the 5' untranslated region of Xenopus c-myc I mRNA during early development
Oncogene, Vol.7(5), pp.1037-1041
05/1992
Handle:
https://hdl.handle.net/2376/113477
PMID: 1570150
Abstract
The translational efficiency of chloramphenicol acetyltransferase (CAT) mRNA containing 5' untranslated region (UTR) sequences of Xenopus c-myc I mRNA was examined in the Xenopus oocyte and early embryo. In contrast to their reduced translation in the oocyte, CAT mRNAs containing 5' UTR sequences located between the c-myc I P0 and P2 promoters translate as efficiently as CAT mRNA controls when injected into early embryos. This pattern of differential regulation of c-myc I translation is similar to that observed for mouse c-myc 5' UTR-containing CAT transcripts during Xenopus oogenesis and early embryogenesis [Lazarus, P., Parkin, N. & Sonenberg, N. (1988). Oncogene, 3, 517-521]. Oocyte-specific translational inhibition was not observed for CAT mRNAs containing c-myc I 5' UTR sequences located 3' of the P2 promoter. No significant alteration in c-myc I 5' UTR/CAT mRNA stability was observed in the oocyte as compared with CAT mRNA controls. The significance of this promoter-dependent translational regulation with respect to c-myc expression and early Xenopus development is discussed.
Metrics
9 Record Views
Details
- Title
- The regulation of translation by the 5' untranslated region of Xenopus c-myc I mRNA during early development
- Creators
- P Lazarus - Department of Biochemistry, McGill University, Montreal, Canada
- Publication Details
- Oncogene, Vol.7(5), pp.1037-1041
- Academic Unit
- Pharmaceutical Sciences, Department of
- Publisher
- England
- Identifiers
- 99900547719301842
- Language
- English
- Resource Type
- Journal article