Journal article
The role of backbone stability near Ala44 in the high reduction potential class of rubredoxins
Proteins, structure, function, and bioinformatics, Vol.62(3), pp.708-714
03/15/2006
Handle:
https://hdl.handle.net/2376/115795
PMID: 16362979
Abstract
Rubredoxins may be separated into high and low reduction potential classes, with reduction potentials differing by approximately 50 mV. Our previous work showed that a local shift in the polar backbone due to an A(44) versus V(44) side-chain size causes this reduction potential difference. However, this work also indicated that in the low potential Clostridium pasteurianum (Cp) rubredoxin, a V(44) --> A(44) mutation causes larger local backbone flexibility, because the V(44) side-chain present in the wild-type (wt) is no longer present to interlock with neighboring residues to stabilize the subsequent G(45). Since Pyrococcus furiosus (Pf) and other high potential rubredoxins generally have a P(45), it was presumed that a G(45) --> P(45) mutation might stabilize a V(44) --> A(44) mutation in Cp rubredoxin. Here crystal structure analysis, energy minimization, and molecular dynamics (MD) were performed for wt V(44)G(45), single mutant A(44)G(45) and double mutant A(44)P(45) Cp, and for wt A(44)P(45) Pf rubredoxins. The local structural, dynamical, and electrostatic properties of Cp gradually approach wt Pf in the order wt Cp to single to double mutant because of greater sequence similarity, as expected. The double mutant A(44)P(45) Cp exhibits increased backbone stability near residue 44 and thus enhances the probability that the backbone dipoles point toward the redox site, which favors an increase in the electrostatic contribution to the reduction potential. It appears that the electrostatic potential of residue 44 and the solvent accessibility to the redox are both determinants for the reduction potentials of homologous rubredoxins. Overall, these results indicate that an A(44) in a rubredoxin may require a P(45) for backbone stability whereas a V(44) can accommodate a G(45), since the valine side-chain can interlock with its neighbors.
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Details
- Title
- The role of backbone stability near Ala44 in the high reduction potential class of rubredoxins
- Creators
- Ming-Liang Tan - Department of Chemistry, Georgetown University, Washington, DC 20057, USAChulHee KangToshiko Ichiye
- Publication Details
- Proteins, structure, function, and bioinformatics, Vol.62(3), pp.708-714
- Academic Unit
- Chemistry, Department of
- Publisher
- United States
- Grant note
- R01-GM45303 / NIGMS NIH HHS
- Identifiers
- 99900548201101842
- Language
- English
- Resource Type
- Journal article