Journal article
The roles of plasticity and evolutionary change in shaping gene expression variation in natural populations of extremophile fish
Molecular ecology, Vol.26(22), pp.6384-6399
11/2017
Handle:
https://hdl.handle.net/2376/104989
PMID: 28926156
Abstract
The notorious plasticity of gene expression responses and the complexity of environmental gradients complicate the identification of adaptive differences in gene regulation among populations. We combined transcriptome analyses in nature with common-garden and exposure experiments to establish cause-effect relationships between the presence of a physiochemical stressor and expression differences, as well as to test how evolutionary change and plasticity interact to shape gene expression variation in natural systems. We studied two evolutionarily independent population pairs of an extremophile fish (Poecilia mexicana) living in toxic, hydrogen sulphide (H
S)-rich springs and adjacent nontoxic habitats and assessed genomewide expression patterns of wild-caught and common-garden-raised individuals exposed to different concentrations of H
S. We found that 7.7% of genes that were differentially expressed between sulphidic and nonsulphidic ecotypes remained differentially expressed in the laboratory, indicating that sources of selection other than H
S-or plastic responses to other environmental factors-contribute substantially to gene expression patterns observed in the wild. Concordantly differentially expressed genes in the wild and the laboratory were primarily associated with H
S detoxification, sulphur processing and metabolic physiology. While shared, ancestral plasticity played a minor role in shaping gene expression variation observed in nature, we documented evidence for evolved population differences in the constitutive expression as well as the H
S inducibility of candidate genes. Mechanisms underlying gene expression variation also varied substantially across the two ecotype pairs. These results provide a springboard for studying evolutionary modifications of gene regulatory mechanisms that underlie expression variation in locally adapted populations.
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Details
- Title
- The roles of plasticity and evolutionary change in shaping gene expression variation in natural populations of extremophile fish
- Creators
- Courtney N Passow - Division of Biology, Kansas State University, Manhattan, KS, USAChathurika Henpita - Department of Integrative Biology, Oklahoma State University, Stillwater, OK, USAJennifer H Shaw - Department of Integrative Biology, Oklahoma State University, Stillwater, OK, USACorey R Quackenbush - School of Biological Sciences, Washington State University, Pullman, WA, USAWesley C Warren - McDonnell Genome Institute, Washington University, St. Louis, MO, USAManfred Schartl - Hagler Institute for Advanced Studies and Department of Biology, Texas A&M University, College Station, TX, USALenin Arias-Rodriguez - División Académica de Ciencias Biológicas, Universidad Juárez Autónoma de Tabasco, Villahermosa, MéxicoJoanna L Kelley - School of Biological Sciences, Washington State University, Pullman, WA, USAMichael Tobler - Division of Biology, Kansas State University, Manhattan, KS, USA
- Publication Details
- Molecular ecology, Vol.26(22), pp.6384-6399
- Academic Unit
- Kelley Lab; Biological Sciences, School of
- Publisher
- England
- Grant note
- R24 OD011198 / NIH HHS
- Identifiers
- 99900546979601842
- Language
- English
- Resource Type
- Journal article